Glucose oxidase directly immobilized onto highly porous gold electrodes for sensing and fuel cell applications

Hendrik Du Toit, Mirella Di Lorenzo

Research output: Contribution to journalArticle

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Abstract

The successful implementation of redox-enzyme electrodes in biosensors and enzymatic biofuel cells has been the subject of extensive research.
For high sensitivity and high energy-conversion efficiency, the effective electron transfer at the protein-electrode interface has a key role. This is difficult to achieve in the case of glucose oxidase, due to the fact that for this enzyme the redox centre is buried inside the structure, far from any feasible electrode binding sites.
This study reports, a simple and rapid methodology for the direct immobilisation of glucose oxidase into highly porous gold electrodes. When the resulting electrode was tested as glucose sensor, a Michaelis-Menten kinetic trend was observed, with a detection limit of 25 μM. The bioelectrode sensitivity, calculated against the superficial surface area of the bioelectrode, was of 22.7± 0.1 μA mM-1cm-2.
This glucose oxidase electrode was also tested as an anode in a glucose/O2 enzymatic biofuel cell, leading to a peak power density of 6 µW cm-2 at a potential of 0.2 V.
LanguageEnglish
Pages86-92
JournalElectrochimica Acta
Volume138
DOIs
StatusPublished - 20 Aug 2014

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Glucose Oxidase
Glucose oxidase
Gold
Fuel cells
Enzymatic fuel cells
Electrodes
Enzyme electrodes
Glucose sensors
Binding sites
Energy conversion
Biosensors
Conversion efficiency
Glucose
Anodes
Enzymes
Binding Sites
Proteins
Kinetics
Electrons

Cite this

Glucose oxidase directly immobilized onto highly porous gold electrodes for sensing and fuel cell applications. / Du Toit, Hendrik; Di Lorenzo, Mirella.

In: Electrochimica Acta, Vol. 138, 20.08.2014, p. 86-92.

Research output: Contribution to journalArticle

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