Genomic variations leading to alterations in cell morphology of Campylobacter spp

Diane Esson, Alison E. Mather, Eoin Scanlan, Srishti Gupta, Stefan P.W. De Vries, David Bailey, Simon R. Harris, Trevelyan J. McKinley, Guillaume Méric, Sophia K. Berry, Pietro Mastroeni, Samuel K. Sheppard, Graham Christie, Nicholas R. Thomson, Julian Parkhill, Duncan J. Maskell, Andrew J. Grant

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. However, it can also adopt straight rod, elongated helical and coccoid forms. Studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. Here, we aimed to determine the genetic factors involved in generating the helical shape of Campylobacter. A C. jejuni transposon (Tn) mutant library was screened for non-helical mutants with inconsistent results. Whole genome sequence variation and morphological trends within this Tn library, and in various C. jejuni wild type strains, were compared and correlated to detect genomic elements associated with helical and rod morphologies. All rod-shaped C. jejuni Tn mutants and all rod-shaped laboratory, clinical and environmental C. jejuni and Campylobacter coli contained genetic changes within the pgp1 or pgp2 genes, which encode peptidoglycan modifying enzymes. We therefore confirm the importance of Pgp1 and Pgp2 in the maintenance of helical shape and extended this to a wide range of C. jejuni and C. coli isolates. Genome sequence analysis revealed variation in the sequence and length of homopolymeric tracts found within these genes, providing a potential mechanism of phase variation of cell shape.

Original languageEnglish
Article number38303
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2 Dec 2016

ASJC Scopus subject areas

  • General

Cite this

Esson, D., Mather, A. E., Scanlan, E., Gupta, S., De Vries, S. P. W., Bailey, D., ... Grant, A. J. (2016). Genomic variations leading to alterations in cell morphology of Campylobacter spp. Scientific Reports, 6, [38303]. https://doi.org/10.1038/srep38303

Genomic variations leading to alterations in cell morphology of Campylobacter spp. / Esson, Diane; Mather, Alison E.; Scanlan, Eoin; Gupta, Srishti; De Vries, Stefan P.W.; Bailey, David; Harris, Simon R.; McKinley, Trevelyan J.; Méric, Guillaume; Berry, Sophia K.; Mastroeni, Pietro; Sheppard, Samuel K.; Christie, Graham; Thomson, Nicholas R.; Parkhill, Julian; Maskell, Duncan J.; Grant, Andrew J.

In: Scientific Reports, Vol. 6, 38303, 02.12.2016.

Research output: Contribution to journalArticle

Esson, D, Mather, AE, Scanlan, E, Gupta, S, De Vries, SPW, Bailey, D, Harris, SR, McKinley, TJ, Méric, G, Berry, SK, Mastroeni, P, Sheppard, SK, Christie, G, Thomson, NR, Parkhill, J, Maskell, DJ & Grant, AJ 2016, 'Genomic variations leading to alterations in cell morphology of Campylobacter spp', Scientific Reports, vol. 6, 38303. https://doi.org/10.1038/srep38303
Esson D, Mather AE, Scanlan E, Gupta S, De Vries SPW, Bailey D et al. Genomic variations leading to alterations in cell morphology of Campylobacter spp. Scientific Reports. 2016 Dec 2;6. 38303. https://doi.org/10.1038/srep38303
Esson, Diane ; Mather, Alison E. ; Scanlan, Eoin ; Gupta, Srishti ; De Vries, Stefan P.W. ; Bailey, David ; Harris, Simon R. ; McKinley, Trevelyan J. ; Méric, Guillaume ; Berry, Sophia K. ; Mastroeni, Pietro ; Sheppard, Samuel K. ; Christie, Graham ; Thomson, Nicholas R. ; Parkhill, Julian ; Maskell, Duncan J. ; Grant, Andrew J. / Genomic variations leading to alterations in cell morphology of Campylobacter spp. In: Scientific Reports. 2016 ; Vol. 6.
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