Genomic Imprinting: Methods and Protocols

Andrew Ward (Editor)

Research output: Book/ReportBook

Abstract

Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Protocols, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of protocols describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results.
Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Protocols brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes.
LanguageEnglish
Place of PublicationNew Jersey
PublisherHumana Press
Volume181
ISBN (Print)978-1-59259-211-1
StatusPublished - 2001

Publication series

NameMethods in Molecular Biology

Fingerprint

Genomic Imprinting
Genes
Organism Cloning
Embryonic Structures
DNA-Binding Proteins
DNA Methylation
Embryonic Stem Cells
Growth and Development
Methylation
Transgenic Mice
Chromatin
Molecular Biology
Neoplasms
Alleles
Research Personnel
Gene Expression
Growth

Cite this

Ward, A. (Ed.) (2001). Genomic Imprinting: Methods and Protocols. (Methods in Molecular Biology). New Jersey: Humana Press.

Genomic Imprinting : Methods and Protocols. / Ward, Andrew (Editor).

New Jersey : Humana Press, 2001. (Methods in Molecular Biology).

Research output: Book/ReportBook

Ward, A (ed.) 2001, Genomic Imprinting: Methods and Protocols. Methods in Molecular Biology, vol. 181, Humana Press, New Jersey.
Ward A, (ed.). Genomic Imprinting: Methods and Protocols. New Jersey: Humana Press, 2001. (Methods in Molecular Biology).
Ward, Andrew (Editor). / Genomic Imprinting : Methods and Protocols. New Jersey : Humana Press, 2001. (Methods in Molecular Biology).
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