Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers

JP Demuth; DW Drury; ML Peters; JD Van Dyken; Nicholas Priest; M J Wade

doi:10.1111/j.1471-8286.2007.01826.x

Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers

JP Demuth, DW Drury, ML Peters, JD Van Dyken, Nicholas Priest, M J Wade

Indiana University Bloomington

Research output: Contribution to journal › Article › peer-review

28 Citations (SciVal)

Abstract

We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies.

Original language	English
Pages (from-to)	1189-1195
Number of pages	7
Journal	Molecular Ecology Notes
Volume	7
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01826.x
Publication status	Published - Nov 2007

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10.1111/j.1471-8286.2007.01826.x

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abstract = "We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies. ",

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AU - Drury, DW

AU - Peters, ML

AU - Van Dyken, JD

AU - Priest, Nicholas

AU - Wade, M J

PY - 2007/11

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AB - We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies.

UR - http://dx.doi.org/10.1111/j.1471-8286.2007.01826.x

U2 - 10.1111/j.1471-8286.2007.01826.x

DO - 10.1111/j.1471-8286.2007.01826.x

M3 - Article

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VL - 7

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EP - 1195

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 6

ER -

Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers

Abstract

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