Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Benjamin Delatte, Jana Jeschke, Matthieu Defrance, Martin Bachman, Catherine Creppe, Emilie Calonne, Martin Bizet, Rachel Deplus, Laura Marroquí, Myriam Libin, Mirunalini Ravichandran, Françoise Mascart, Decio L. Eizirik, Adele Murrell, Tomasz P. Jurkowski, François Fuks

Research output: Contribution to journalArticlepeer-review

47 Citations (SciVal)

Abstract

The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

Original languageEnglish
Article number12714
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 4 Aug 2015

Bibliographical note

Funding Information:
BD, JJ, CC, RD are supported by the Belgian FNRS. MD is supported by Innoviris. MB is supported by the Televie. FF is an FNRS ‘Senior Research Associate’. BD is an ‘FNRS Aspirant’. FF’s laboratory was funded by grants from the FNRS and Televie, the ‘Interuniversity Attraction Poles’ (IAP Phase VII no P7/03) and by the ‘Action de Recherche Concerté’ (AUWB-2010-2015 ULB-No 7). We thank Dr. F.F Chu for providing the GPx1/2 Dko and wt genomic DNA extracts.

ASJC Scopus subject areas

  • General

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