@article{8dd031025b694f91acbbb5ddfbed5df6,
title = "Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress",
abstract = "The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.",
author = "Benjamin Delatte and Jana Jeschke and Matthieu Defrance and Martin Bachman and Catherine Creppe and Emilie Calonne and Martin Bizet and Rachel Deplus and Laura Marroqu{\'i} and Myriam Libin and Mirunalini Ravichandran and Fran{\c c}oise Mascart and Eizirik, {Decio L.} and Adele Murrell and Jurkowski, {Tomasz P.} and Fran{\c c}ois Fuks",
note = "Funding Information: BD, JJ, CC, RD are supported by the Belgian FNRS. MD is supported by Innoviris. MB is supported by the Televie. FF is an FNRS {\textquoteleft}Senior Research Associate{\textquoteright}. BD is an {\textquoteleft}FNRS Aspirant{\textquoteright}. FF{\textquoteright}s laboratory was funded by grants from the FNRS and Televie, the {\textquoteleft}Interuniversity Attraction Poles{\textquoteright} (IAP Phase VII no P7/03) and by the {\textquoteleft}Action de Recherche Concert{\'e}{\textquoteright} (AUWB-2010-2015 ULB-No 7). We thank Dr. F.F Chu for providing the GPx1/2 Dko and wt genomic DNA extracts.",
year = "2015",
month = aug,
day = "4",
doi = "10.1038/srep12714",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",
}