Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Benjamin Delatte; Jana Jeschke; Matthieu Defrance; Martin Bachman; Catherine Creppe; Emilie Calonne; Martin Bizet; Rachel Deplus; Laura Marroquí; Myriam Libin; Mirunalini Ravichandran; Françoise Mascart; Decio L. Eizirik; Adele Murrell; Tomasz P. Jurkowski; François Fuks

doi:10.1038/srep12714

Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Benjamin Delatte, Jana Jeschke, Matthieu Defrance, Martin Bachman, Catherine Creppe, Emilie Calonne, Martin Bizet, Rachel Deplus, Laura Marroquí, Myriam Libin, Mirunalini Ravichandran, Françoise Mascart, Decio L. Eizirik, Adele Murrell, Tomasz P. Jurkowski, François Fuks

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50 Citations (SciVal)

Abstract

The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

Original language	English
Article number	12714
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep12714
Publication status	Published - 4 Aug 2015

Bibliographical note

Funding Information:
BD, JJ, CC, RD are supported by the Belgian FNRS. MD is supported by Innoviris. MB is supported by the Televie. FF is an FNRS ‘Senior Research Associate’. BD is an ‘FNRS Aspirant’. FF’s laboratory was funded by grants from the FNRS and Televie, the ‘Interuniversity Attraction Poles’ (IAP Phase VII no P7/03) and by the ‘Action de Recherche Concerté’ (AUWB-2010-2015 ULB-No 7). We thank Dr. F.F Chu for providing the GPx1/2 Dko and wt genomic DNA extracts.

Funding

BD, JJ, CC, RD are supported by the Belgian FNRS. MD is supported by Innoviris. MB is supported by the Televie. FF is an FNRS ‘Senior Research Associate’. BD is an ‘FNRS Aspirant’. FF’s laboratory was funded by grants from the FNRS and Televie, the ‘Interuniversity Attraction Poles’ (IAP Phase VII no P7/03) and by the ‘Action de Recherche Concerté’ (AUWB-2010-2015 ULB-No 7). We thank Dr. F.F Chu for providing the GPx1/2 Dko and wt genomic DNA extracts.

ASJC Scopus subject areas

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10.1038/srep12714Licence: CC BY

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Delatte, B., Jeschke, J., Defrance, M., Bachman, M., Creppe, C., Calonne, E., Bizet, M., Deplus, R., Marroquí, L., Libin, M., Ravichandran, M., Mascart, F., Eizirik, D. L., Murrell, A., Jurkowski, T. P., & Fuks, F. (2015). Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress. Scientific Reports, 5, Article 12714. https://doi.org/10.1038/srep12714

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abstract = "The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.",

author = "Benjamin Delatte and Jana Jeschke and Matthieu Defrance and Martin Bachman and Catherine Creppe and Emilie Calonne and Martin Bizet and Rachel Deplus and Laura Marroqu{\'i} and Myriam Libin and Mirunalini Ravichandran and Fran{\c c}oise Mascart and Eizirik, {Decio L.} and Adele Murrell and Jurkowski, {Tomasz P.} and Fran{\c c}ois Fuks",

note = "Funding Information: BD, JJ, CC, RD are supported by the Belgian FNRS. MD is supported by Innoviris. MB is supported by the Televie. FF is an FNRS {\textquoteleft}Senior Research Associate{\textquoteright}. BD is an {\textquoteleft}FNRS Aspirant{\textquoteright}. FF{\textquoteright}s laboratory was funded by grants from the FNRS and Televie, the {\textquoteleft}Interuniversity Attraction Poles{\textquoteright} (IAP Phase VII no P7/03) and by the {\textquoteleft}Action de Recherche Concert{\'e}{\textquoteright} (AUWB-2010-2015 ULB-No 7). We thank Dr. F.F Chu for providing the GPx1/2 Dko and wt genomic DNA extracts.",

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AU - Bizet, Martin

AU - Deplus, Rachel

AU - Marroquí, Laura

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AU - Ravichandran, Mirunalini

AU - Mascart, Françoise

AU - Eizirik, Decio L.

AU - Murrell, Adele

AU - Jurkowski, Tomasz P.

AU - Fuks, François

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Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Abstract

Bibliographical note

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