Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Benjamin Delatte, Jana Jeschke, Matthieu Defrance, Martin Bachman, Catherine Creppe, Emilie Calonne, Martin Bizet, Rachel Deplus, Laura Marroquí, Myriam Libin, Mirunalini Ravichandran, Françoise Mascart, Decio L. Eizirik, Adele Murrell, Tomasz P. Jurkowski, François Fuks

Research output: Contribution to journalArticlepeer-review

31 Citations (SciVal)


The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

Original languageEnglish
Article number12714
JournalScientific Reports
Publication statusPublished - 4 Aug 2015

ASJC Scopus subject areas

  • General


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