Genome-wide association study identifies vitamin B5 biosynthesis as a host specificity factor in Campylobacter

Samuel K. Sheppard, Xavier Didelot, Guillaume Meric, Alicia Torralbo, Keith A. Jolley, David J Kelly, Stephen D Bentley, Martin C J Maiden, Julian Parkhill, Daniel Falush

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Genome-wide association studies have the potential to identify causal genetic factors underlying important phenotypes but have rarely been performed in bacteria. We present an association mapping method that takes into account the clonal population structure of bacteria and is applicable to both core and accessory genome variation. Campylobacter is a common cause of human gastroenteritis as a consequence of its proliferation in multiple farm animal species and its transmission via contaminated meat and poultry. We applied our association mapping method to identify the factors responsible for adaptation to cattle and chickens among 192 Campylobacter isolates from these and other host sources. Phylogenetic analysis implied frequent host switching but also showed that some lineages were strongly associated with particular hosts. A seven-gene region with a host association signal was found. Genes in this region were almost universally present in cattle but were frequently absent in isolates from chickens and wild birds. Three of the seven genes encoded vitamin B5 biosynthesis. We found that isolates from cattle were better able to grow in vitamin B5-depleted media and propose that this difference may be an adaptation to host diet.

Original languageEnglish
Pages (from-to)11923-11927
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number29
DOIs
Publication statusPublished - 16 Jul 2013

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Pantothenic Acid
Campylobacter
Genome-Wide Association Study
Host Specificity
Chickens
Genes
Bacteria
Domestic Animals
Gastroenteritis
Poultry
Meat
Birds
Genome
Diet
Phenotype
Population

Keywords

  • Evolution
  • Genomics
  • Host Adaptation
  • Transmission Ecology

Cite this

Genome-wide association study identifies vitamin B5 biosynthesis as a host specificity factor in Campylobacter. / Sheppard, Samuel K.; Didelot, Xavier; Meric, Guillaume; Torralbo, Alicia; Jolley, Keith A.; Kelly, David J; Bentley, Stephen D; Maiden, Martin C J; Parkhill, Julian; Falush, Daniel.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 29, 16.07.2013, p. 11923-11927.

Research output: Contribution to journalArticle

Sheppard, Samuel K. ; Didelot, Xavier ; Meric, Guillaume ; Torralbo, Alicia ; Jolley, Keith A. ; Kelly, David J ; Bentley, Stephen D ; Maiden, Martin C J ; Parkhill, Julian ; Falush, Daniel. / Genome-wide association study identifies vitamin B5 biosynthesis as a host specificity factor in Campylobacter. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 29. pp. 11923-11927.
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