Genome-wide analysis of alternative splicing evolution among Mus subspecies

Bettina Harr, Leslie M. Turner

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Alternative splicing, the combination of different exons to produce a variety of transcripts from a single gene, contributes enormously to transcriptome diversity in mammals, and the majority of genes encode alternatively spliced products. Previous research comparing mouse, rat and human has shown that a significant proportion of splice forms are not conserved across species, suggesting that alternative transcripts are an important source of evolutionary novelty. Here, we studied the evolution of alternative splicing in the early stages of species divergence in the house mouse. We sequenced the testis transcriptomes of three Mus musculus subspecies and Mus spretus using Illumina technology. On the basis of a genome-wide analysis of read coverage differences among subspecies, we identified several hundred candidate alternatively spliced regions. We conservatively estimate that 6.5% of testis-expressed genes show alternative splice differences between at least one pair of M. musculus subspecies, a proportion slightly higher than the proportion of genes differentially expressed among subspecies. These results suggest that differences in both the structure and abundance of transcripts contribute to early transcriptome divergence.

Original languageEnglish
Pages (from-to)228-239
Number of pages12
JournalMolecular Ecology
Volume19
Issue numberSuppl 1
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Mus
alternative splicing
Alternative Splicing
subspecies
Mus musculus
genome
transcriptome
Genome
Transcriptome
gene
testes
genes
divergence
Genes
Testis
application coverage
exons
mammal
mammals
Mammals

Keywords

  • Alternative Splicing
  • Animals
  • Evolution, Molecular
  • Exons
  • Gene Expression Profiling
  • Male
  • Mice
  • Sequence Analysis, DNA
  • Testis
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Genome-wide analysis of alternative splicing evolution among Mus subspecies. / Harr, Bettina; Turner, Leslie M.

In: Molecular Ecology, Vol. 19, No. Suppl 1, 03.2010, p. 228-239.

Research output: Contribution to journalArticle

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