Projects per year
Abstract
Parageobacillus thermoglucosidasius represents a thermophilic, facultative anaerobic bacterial chassis, with several desirable traits for metabolic engineering and industrial production. To further optimize strain productivity, a systems level understanding of its metabolism is needed, which can be facilitated by a genome-scale metabolic model. Here, we present p-thermo, the most complete, curated and validated genome-scale model (to date) of Parageobacillus thermoglucosidasius NCIMB 11955. It spans a total of 890 metabolites, 1175 reactions and 917 metabolic genes, forming an extensive knowledge base for P. thermoglucosidasius NCIMB 11955 metabolism. The model accurately predicts aerobic utilization of 22 carbon sources, and the predictive quality of internal fluxes was validated with previously published 13C-fluxomics data. In an application case, p-thermo was used to facilitate more in-depth analysis of reported metabolic engineering efforts, giving additional insight into fermentative metabolism. Finally, p-thermo was used to resolve a previously uncharacterised bottleneck in anaerobic metabolism, by identifying the minimal required supplemented nutrients (thiamin, biotin and iron(III)) needed to sustain anaerobic growth. This highlights the usefulness of p-thermo for guiding the generation of experimental hypotheses and for facilitating data-driven metabolic engineering, expanding the use of P. thermoglucosidasius as a high yield production platform.
Original language | English |
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Pages (from-to) | 123-134 |
Number of pages | 12 |
Journal | Metabolic Engineering |
Volume | 65 |
Early online date | 19 Mar 2021 |
DOIs | |
Publication status | Published - 31 May 2021 |
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Dive into the research topics of 'Genome-scale metabolic modeling of P. thermoglucosidasius NCIMB 11955 reveals metabolic bottlenecks in anaerobic metabolism'. Together they form a unique fingerprint.Projects
- 2 Finished
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An Integrated Approach to Explore a Novel Paradigm for Biofuel Production from Lignocellulosic Feedstocks
Arnot, T. (PI), Leak, D. (PI), Coma Bech, M. (CoI), Crennell, S. (CoI) & Henk, D. (CoI)
Biotechnology and Biological Sciences Research Council
1/03/17 → 31/03/22
Project: Research council
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David Leak - Development of Geobacillus Thermoglucosidasis as a Robust Platform for Production of Chemicals from Renewables
Leak, D. (PI)
Biotechnology and Biological Sciences Research Council
16/04/12 → 15/10/15
Project: Research council