Genetic, phenotypic and ecological differentiation suggests incipient speciation in two Charadrius plovers along the Chinese coast

Xuejing Wang, Pinjia Que, Gerald Heckel, Junhua Hu, Xuecong Zhang, Chung Yu Chiang, Nan Zhang, Qin Huang, Simin Liu, Jonathan Martinez, Emilio Pagani-Núñez, Caroline Dingle, Yu Yan Leung, Tamás Székely, Zhengwang Zhang, Yang Liu

Research output: Contribution to journalArticle

Abstract

Background: Speciation with gene flow is an alternative to the nascence of new taxa in strict allopatric separation. Indeed, many taxa have parapatric distributions at present. It is often unclear if these are secondary contacts, e.g. caused by past glaciation cycles or the manifestation of speciation with gene flow, which hampers our understanding of how different forces drive diversification. Here we studied genetic, phenotypic and ecological aspects of divergence in a pair of incipient shorebird species, the Kentish (Charadrius alexandrinus) and the White-faced Plovers (C. dealbatus), shorebirds with parapatric breeding ranges along the Chinese coast. We assessed divergence based on molecular markers with different modes of inheritance and quantified phenotypic and ecological divergence in aspects of morphometric, dietary and climatic niches. Results: Our integrative analyses revealed small to moderate levels of genetic and phenotypic distinctiveness with symmetric gene flow across the contact area at the Chinese coast. The two species diverged approximately half a million years ago in dynamic isolation with secondary contact occurring due to cycling sea level changes between the Eastern and Southern China Sea in the mid-late Pleistocene. We found evidence of character displacement and ecological niche differentiation between the two species, invoking the role of selection in facilitating divergence despite gene flow. Conclusion: These findings imply that ecology can indeed counter gene flow through divergent selection and thus contributes to incipient speciation in these plovers. Furthermore, our study highlights the importance of using integrative datasets to reveal the evolutionary history and assist the inference of mechanisms of speciation.

Original languageEnglish
Article number135
JournalBMC Evolutionary Biology
Volume19
Issue number1
DOIs
Publication statusPublished - 27 Jun 2019

Keywords

  • Character displacement
  • Ecological niche
  • Gene flow
  • Hybridization
  • Parapatry
  • Stable isotope analysis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Genetic, phenotypic and ecological differentiation suggests incipient speciation in two Charadrius plovers along the Chinese coast. / Wang, Xuejing; Que, Pinjia; Heckel, Gerald; Hu, Junhua; Zhang, Xuecong; Chiang, Chung Yu; Zhang, Nan; Huang, Qin; Liu, Simin; Martinez, Jonathan; Pagani-Núñez, Emilio; Dingle, Caroline; Leung, Yu Yan; Székely, Tamás; Zhang, Zhengwang; Liu, Yang.

In: BMC Evolutionary Biology, Vol. 19, No. 1, 135, 27.06.2019.

Research output: Contribution to journalArticle

Wang, X, Que, P, Heckel, G, Hu, J, Zhang, X, Chiang, CY, Zhang, N, Huang, Q, Liu, S, Martinez, J, Pagani-Núñez, E, Dingle, C, Leung, YY, Székely, T, Zhang, Z & Liu, Y 2019, 'Genetic, phenotypic and ecological differentiation suggests incipient speciation in two Charadrius plovers along the Chinese coast', BMC Evolutionary Biology, vol. 19, no. 1, 135. https://doi.org/10.1186/s12862-019-1449-5
Wang, Xuejing ; Que, Pinjia ; Heckel, Gerald ; Hu, Junhua ; Zhang, Xuecong ; Chiang, Chung Yu ; Zhang, Nan ; Huang, Qin ; Liu, Simin ; Martinez, Jonathan ; Pagani-Núñez, Emilio ; Dingle, Caroline ; Leung, Yu Yan ; Székely, Tamás ; Zhang, Zhengwang ; Liu, Yang. / Genetic, phenotypic and ecological differentiation suggests incipient speciation in two Charadrius plovers along the Chinese coast. In: BMC Evolutionary Biology. 2019 ; Vol. 19, No. 1.
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AU - Wang, Xuejing

AU - Que, Pinjia

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AU - Hu, Junhua

AU - Zhang, Xuecong

AU - Chiang, Chung Yu

AU - Zhang, Nan

AU - Huang, Qin

AU - Liu, Simin

AU - Martinez, Jonathan

AU - Pagani-Núñez, Emilio

AU - Dingle, Caroline

AU - Leung, Yu Yan

AU - Székely, Tamás

AU - Zhang, Zhengwang

AU - Liu, Yang

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Y1 - 2019/6/27

N2 - Background: Speciation with gene flow is an alternative to the nascence of new taxa in strict allopatric separation. Indeed, many taxa have parapatric distributions at present. It is often unclear if these are secondary contacts, e.g. caused by past glaciation cycles or the manifestation of speciation with gene flow, which hampers our understanding of how different forces drive diversification. Here we studied genetic, phenotypic and ecological aspects of divergence in a pair of incipient shorebird species, the Kentish (Charadrius alexandrinus) and the White-faced Plovers (C. dealbatus), shorebirds with parapatric breeding ranges along the Chinese coast. We assessed divergence based on molecular markers with different modes of inheritance and quantified phenotypic and ecological divergence in aspects of morphometric, dietary and climatic niches. Results: Our integrative analyses revealed small to moderate levels of genetic and phenotypic distinctiveness with symmetric gene flow across the contact area at the Chinese coast. The two species diverged approximately half a million years ago in dynamic isolation with secondary contact occurring due to cycling sea level changes between the Eastern and Southern China Sea in the mid-late Pleistocene. We found evidence of character displacement and ecological niche differentiation between the two species, invoking the role of selection in facilitating divergence despite gene flow. Conclusion: These findings imply that ecology can indeed counter gene flow through divergent selection and thus contributes to incipient speciation in these plovers. Furthermore, our study highlights the importance of using integrative datasets to reveal the evolutionary history and assist the inference of mechanisms of speciation.

AB - Background: Speciation with gene flow is an alternative to the nascence of new taxa in strict allopatric separation. Indeed, many taxa have parapatric distributions at present. It is often unclear if these are secondary contacts, e.g. caused by past glaciation cycles or the manifestation of speciation with gene flow, which hampers our understanding of how different forces drive diversification. Here we studied genetic, phenotypic and ecological aspects of divergence in a pair of incipient shorebird species, the Kentish (Charadrius alexandrinus) and the White-faced Plovers (C. dealbatus), shorebirds with parapatric breeding ranges along the Chinese coast. We assessed divergence based on molecular markers with different modes of inheritance and quantified phenotypic and ecological divergence in aspects of morphometric, dietary and climatic niches. Results: Our integrative analyses revealed small to moderate levels of genetic and phenotypic distinctiveness with symmetric gene flow across the contact area at the Chinese coast. The two species diverged approximately half a million years ago in dynamic isolation with secondary contact occurring due to cycling sea level changes between the Eastern and Southern China Sea in the mid-late Pleistocene. We found evidence of character displacement and ecological niche differentiation between the two species, invoking the role of selection in facilitating divergence despite gene flow. Conclusion: These findings imply that ecology can indeed counter gene flow through divergent selection and thus contributes to incipient speciation in these plovers. Furthermore, our study highlights the importance of using integrative datasets to reveal the evolutionary history and assist the inference of mechanisms of speciation.

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KW - Ecological niche

KW - Gene flow

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KW - Parapatry

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