Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida

Shradha Nirwan; Anupriya Chatterjee; Volkan Cevik; Eric B. Holub; Jonathan D.G. Jones; Anand Kumar Tewari; Neeraj Shrivastava; Abha Agnihotri; Pankaj Sharma

doi:10.1007/s11033-023-09040-w

Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida

Shradha Nirwan, Anupriya Chatterjee, Volkan Cevik, Eric B. Holub, Jonathan D.G. Jones, Anand Kumar Tewari, Neeraj Shrivastava, Abha Agnihotri, Pankaj Sharma

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Brassica species is the second most important edible oilseed crop in India. Albugo candida (Pers.) Kuntze, a major oomycete disease of oilseed brassica causing white rust, leads to 60% yield loss globally. The prevalence of A. candida race 2 (Ac2V) that specifically infects B. juncea, coupled with limitations of conventional methods has resulted in a dearth of white rust resistance resources in cultivated varieties. Methods and results: In an effort to develop resistant plants, Agrobacterium mediated genetic transformation of three B. juncea genotypes viz., susceptible host var. Varuna, along with its doubled haploid mutant lines C66 and C69 (showing moderate tolerance to field isolates of A. candida) was initiated to transfer resistance genes (WRR8^Sf-2 and WRR9^Hi-0) identified in Arabidopsis thaliana against race Ac2V, that encode for Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat proteins that recognize effectors of the pathogen races. Conclusions: Our results demonstrate that introduction of resistance genes from a tertiary gene pool by genetic transformation enhances disease resistance in B. juncea genotypes to a highly virulent Ac2V isolate.

Original language	English
Article number	199
Journal	Molecular Biology Reports
Volume	51
Issue number	1
Early online date	25 Jan 2024
DOIs	https://doi.org/10.1007/s11033-023-09040-w
Publication status	Published - 25 Jan 2024

Bibliographical note

Publisher Copyright:
© 2024, The Author(s), under exclusive licence to Springer Nature B.V.

Funding

We are grateful to Prof. Deepak Pental and Prof. MV Rajam, University of Delhi South Campus, New Delhi and their associates for help in genetic transformation and molecular work. Authors are thankful to the India Department of Biotechnology (DBT) and the UK Biotechnology and Biological Sciences Research Council (BBSRC) for joint funding of this collaborative work as a Crop Genomics and Technologies (CGAT) project: DBT funding BT/IN/Indo-UK/CGAT/12/DP/2014-2015 & BBSRC funding BB/L011646/1.

Funders	Funder number
University of Delhi South Campus
Biotechnology and Biological Sciences Research Council	BB/L011646/1
Department of Biotechnology, Ministry of Science and Technology, India

Keywords

Agrobacterium-mediated transformation
Albugo candida
Brassica juncea
White rust

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/s11033-023-09040-w

Cite this

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abstract = "Background: Brassica species is the second most important edible oilseed crop in India. Albugo candida (Pers.) Kuntze, a major oomycete disease of oilseed brassica causing white rust, leads to 60% yield loss globally. The prevalence of A. candida race 2 (Ac2V) that specifically infects B. juncea, coupled with limitations of conventional methods has resulted in a dearth of white rust resistance resources in cultivated varieties. Methods and results: In an effort to develop resistant plants, Agrobacterium mediated genetic transformation of three B. juncea genotypes viz., susceptible host var. Varuna, along with its doubled haploid mutant lines C66 and C69 (showing moderate tolerance to field isolates of A. candida) was initiated to transfer resistance genes (WRR8Sf-2 and WRR9Hi-0) identified in Arabidopsis thaliana against race Ac2V, that encode for Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat proteins that recognize effectors of the pathogen races. Conclusions: Our results demonstrate that introduction of resistance genes from a tertiary gene pool by genetic transformation enhances disease resistance in B. juncea genotypes to a highly virulent Ac2V isolate.",

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Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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