Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Xiaomei Yan, Zongwei Li, Monika A Chlebowicz, Xiaoxia Tao, Ming Ni, Yuan Hu, Zhen Li, Hajo Grundmann, Susan Murray, Ben Pascoe, Samuel K Sheppard, Xiaochen Bo, Jan Maarten van Dijl, Pengcheng Du, Minli Zhang, Yuanhai You, Xiaojie Yu, Fanliang Meng, Shengqi Wang, Jianzhong Zhang

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Abstract

Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

Original languageEnglish
Pages (from-to)722–729
JournalInternational Journal of Medical Microbiology
Volume306
Issue number8
Early online date10 Aug 2016
DOIs
Publication statusPublished - Dec 2016

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