Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Xiaomei Yan; Zongwei Li; Monika A Chlebowicz; Xiaoxia Tao; Ming Ni; Yuan Hu; Zhen Li; Hajo Grundmann; Susan Murray; Ben Pascoe; Samuel K Sheppard; Xiaochen Bo; Jan Maarten van Dijl; Pengcheng Du; Minli Zhang; Yuanhai You; Xiaojie Yu; Fanliang Meng; Shengqi Wang; Jianzhong Zhang

doi:10.1016/j.ijmm.2016.08.001

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Xiaomei Yan, Zongwei Li, Monika A Chlebowicz, Xiaoxia Tao, Ming Ni, Yuan Hu, Zhen Li, Hajo Grundmann, Susan Murray, Ben Pascoe, Samuel K Sheppard, Xiaochen Bo, Jan Maarten van Dijl, Pengcheng Du, Minli Zhang, Yuanhai You, Xiaojie Yu, Fanliang Meng, Shengqi Wang, Jianzhong Zhang

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Abstract

Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

Original language	English
Pages (from-to)	722–729
Journal	International Journal of Medical Microbiology
Volume	306
Issue number	8
Early online date	10 Aug 2016
DOIs	https://doi.org/10.1016/j.ijmm.2016.08.001
Publication status	Published - Dec 2016

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10.1016/j.ijmm.2016.08.001

2016 Yan et al ST9_IJMMAccepted author manuscript, 1.73 MBLicence: CC BY-NC-ND

http://dx.doi.org/10.1016/j.ijmm.2016.08.001

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Yan, X., Li, Z., Chlebowicz, M. A., Tao, X., Ni, M., Hu, Y., Li, Z., Grundmann, H., Murray, S., Pascoe, B., Sheppard, S. K., Bo, X., Dijl, J. M. V., Du, P., Zhang, M., You, Y., Yu, X., Meng, F., Wang, S., & Zhang, J. (2016). Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance. International Journal of Medical Microbiology, 306(8), 722–729. https://doi.org/10.1016/j.ijmm.2016.08.001

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance. / Yan, Xiaomei; Li, Zongwei; Chlebowicz, Monika A et al.
In: International Journal of Medical Microbiology, Vol. 306, No. 8, 12.2016, p. 722–729.

Research output: Contribution to journal › Article › peer-review

Yan, X, Li, Z, Chlebowicz, MA, Tao, X, Ni, M, Hu, Y, Li, Z, Grundmann, H, Murray, S, Pascoe, B, Sheppard, SK, Bo, X, Dijl, JMV, Du, P, Zhang, M, You, Y, Yu, X, Meng, F, Wang, S & Zhang, J 2016, 'Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance', International Journal of Medical Microbiology, vol. 306, no. 8, pp. 722–729. https://doi.org/10.1016/j.ijmm.2016.08.001

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title = "Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance",

abstract = "Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.",

author = "Xiaomei Yan and Zongwei Li and Chlebowicz, {Monika A} and Xiaoxia Tao and Ming Ni and Yuan Hu and Zhen Li and Hajo Grundmann and Susan Murray and Ben Pascoe and Sheppard, {Samuel K} and Xiaochen Bo and Dijl, {Jan Maarten van} and Pengcheng Du and Minli Zhang and Yuanhai You and Xiaojie Yu and Fanliang Meng and Shengqi Wang and Jianzhong Zhang",

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doi = "10.1016/j.ijmm.2016.08.001",

language = "English",

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AU - Li, Zongwei

AU - Chlebowicz, Monika A

AU - Tao, Xiaoxia

AU - Ni, Ming

AU - Hu, Yuan

AU - Li, Zhen

AU - Grundmann, Hajo

AU - Murray, Susan

AU - Pascoe, Ben

AU - Sheppard, Samuel K

AU - Bo, Xiaochen

AU - Dijl, Jan Maarten van

AU - Du, Pengcheng

AU - Zhang, Minli

AU - You, Yuanhai

AU - Yu, Xiaojie

AU - Meng, Fanliang

AU - Wang, Shengqi

AU - Zhang, Jianzhong

PY - 2016/12

Y1 - 2016/12

N2 - Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

AB - Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

UR - http://dx.doi.org/10.1016/j.ijmm.2016.08.001

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DO - 10.1016/j.ijmm.2016.08.001

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SN - 1438-4221

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JO - International Journal of Medical Microbiology

JF - International Journal of Medical Microbiology

IS - 8

ER -

Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

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