Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 3763-3767 |
| Number of pages | 5 |
| Journal | Infection and immunity |
| Volume | 67 |
| Issue number | 8 |
| Publication status | Published - 1999 |
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Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. / Preston, A; Allen, A G; Cadisch, J; Thomas, R; Stevens, K; Churcher, C M; Badcock, K L; Parkhill, J; Barrell, B; Maskell, D J.
In: Infection and immunity, Vol. 67, No. 8, 1999, p. 3763-3767.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae
AU - Preston, A
AU - Allen, A G
AU - Cadisch, J
AU - Thomas, R
AU - Stevens, K
AU - Churcher, C M
AU - Badcock, K L
AU - Parkhill, J
AU - Barrell, B
AU - Maskell, D J
PY - 1999
Y1 - 1999
N2 - Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.
AB - Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.
UR - http://iai.asm.org/content/67/8/3763
M3 - Article
C2 - 10417135
VL - 67
SP - 3763
EP - 3767
JO - Infection and immunity
JF - Infection and immunity
SN - 0019-9567
IS - 8
ER -