Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

A Preston, A G Allen, J Cadisch, R Thomas, K Stevens, C M Churcher, K L Badcock, J Parkhill, B Barrell, D J Maskell

Research output: Contribution to journalArticle

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Abstract

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.
Original languageEnglish
Pages (from-to)3763-3767
Number of pages5
JournalInfection and immunity
Volume67
Issue number8
Publication statusPublished - 1999

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O Antigens
Bordetella bronchiseptica
Bordetella parapertussis
Lipopolysaccharides
Bordetella pertussis
Open Reading Frames
Genetic Loci
Insertional Mutagenesis
Polysaccharides
Databases
Bacteria
Mutation
Infection

Cite this

Preston, A., Allen, A. G., Cadisch, J., Thomas, R., Stevens, K., Churcher, C. M., ... Maskell, D. J. (1999). Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. Infection and immunity, 67(8), 3763-3767.

Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. / Preston, A; Allen, A G; Cadisch, J; Thomas, R; Stevens, K; Churcher, C M; Badcock, K L; Parkhill, J; Barrell, B; Maskell, D J.

In: Infection and immunity, Vol. 67, No. 8, 1999, p. 3763-3767.

Research output: Contribution to journalArticle

Preston, A, Allen, AG, Cadisch, J, Thomas, R, Stevens, K, Churcher, CM, Badcock, KL, Parkhill, J, Barrell, B & Maskell, DJ 1999, 'Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae', Infection and immunity, vol. 67, no. 8, pp. 3763-3767.
Preston A, Allen AG, Cadisch J, Thomas R, Stevens K, Churcher CM et al. Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. Infection and immunity. 1999;67(8):3763-3767.
Preston, A ; Allen, A G ; Cadisch, J ; Thomas, R ; Stevens, K ; Churcher, C M ; Badcock, K L ; Parkhill, J ; Barrell, B ; Maskell, D J. / Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. In: Infection and immunity. 1999 ; Vol. 67, No. 8. pp. 3763-3767.
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