Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

A Preston; A G Allen; J Cadisch; R Thomas; K Stevens; C M Churcher; K L Badcock; J Parkhill; B Barrell; D J Maskell

Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

A Preston, A G Allen, J Cadisch, R Thomas, K Stevens, C M Churcher, K L Badcock, J Parkhill, B Barrell, D J Maskell

Department of Biology & Biochemistry

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.

Original language	English
Pages (from-to)	3763-3767
Number of pages	5
Journal	Infection and immunity
Volume	67
Issue number	8
Publication status	Published - 1999

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title = "Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae",

abstract = "Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.",

author = "A Preston and Allen, {A G} and J Cadisch and R Thomas and K Stevens and Churcher, {C M} and Badcock, {K L} and J Parkhill and B Barrell and Maskell, {D J}",

year = "1999",

language = "English",

volume = "67",

pages = "3763--3767",

journal = "Infection and immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "8",

}

TY - JOUR

T1 - Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

AU - Preston, A

AU - Allen, A G

AU - Cadisch, J

AU - Thomas, R

AU - Stevens, K

AU - Churcher, C M

AU - Badcock, K L

AU - Parkhill, J

AU - Barrell, B

AU - Maskell, D J

PY - 1999

Y1 - 1999

N2 - Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.

AB - Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.

UR - http://iai.asm.org/content/67/8/3763

M3 - Article

C2 - 10417135

VL - 67

SP - 3763

EP - 3767

JO - Infection and immunity

JF - Infection and immunity

SN - 0019-9567

IS - 8

ER -