Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes

M C Mullins, M Hammerschmidt, D A Kane, J Odenthal, M Brand, F J M vanEeden, Makoto Furutani-Seiki, M Granato, P Haffter, C P Heisenberg, Y J Jiang, R N Kelsh, C NussleinVolhard

Research output: Contribution to journalArticle

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Abstract

We identified 6 genes that are essential for specifying ventral regions of the early zebrafish embryo, Mutations in these genes cause an expansion of structures normally derived from dorsal-lateral regions of the blastula at the expense of ventrally derived structures, A series of phenotypes of varied strengths is observed with different alleles of these mutants, The weakest phenotype is a reduction in the ventral tail fin, observed as a dominant phenotype of swirl, piggytail, and somitabun and a recessive phenotype of min fin, lost-a-fin and some piggytail alleles, With increasing phenotypic strength, the blood and pronephric anlagen are also reduced or absent, while the paraxial mesoderm and anterior neuroectoderm is progressively expanded, In the strong phenotypes, displayed by homozygous embryos of snailhouse, swirl and somitabun, the somites circle around the embryo and the midbrain region is expanded laterally, Several mutations in this group of genes are semidominant as well as recessive indicating a strong dosage sensitivity of the processes involved, Mutations in the piggytail gene display an unusual dominance that depends on both a maternal and zygotic heterozygous genotype, while somitabun is a fully penetrant dominant maternal-effect mutation, The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages, This pathway provides ventral positional information, counteracting the dorsalizing instructions of the organizer, which is localized in the dorsal shield.
LanguageEnglish
Pages81-93
Number of pages13
JournalDevelopment
Volume123
StatusPublished - 1996

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Zebrafish
Embryonic Structures
Phenotype
Genes
Blastula
Mutation
Alleles
Neural Plate
Oogenesis
Somites
Essential Genes
Mesoderm
Mesencephalon
Tail
Genotype
Mothers

Keywords

  • maternal effect
  • tailbud
  • pattern
  • gastrulation
  • dorsoventral axis
  • zebrafish
  • dorsalized mutant
  • ventral determination
  • formation

Cite this

Mullins, M. C., Hammerschmidt, M., Kane, D. A., Odenthal, J., Brand, M., vanEeden, F. J. M., ... NussleinVolhard, C. (1996). Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes.

Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. / Mullins, M C; Hammerschmidt, M; Kane, D A; Odenthal, J; Brand, M; vanEeden, F J M; Furutani-Seiki, Makoto; Granato, M; Haffter, P; Heisenberg, C P; Jiang, Y J; Kelsh, R N; NussleinVolhard, C.

In: Development, Vol. 123, 1996, p. 81-93.

Research output: Contribution to journalArticle

Mullins, MC, Hammerschmidt, M, Kane, DA, Odenthal, J, Brand, M, vanEeden, FJM, Furutani-Seiki, M, Granato, M, Haffter, P, Heisenberg, CP, Jiang, YJ, Kelsh, RN & NussleinVolhard, C 1996, 'Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes' Development, vol. 123, pp. 81-93.
Mullins MC, Hammerschmidt M, Kane DA, Odenthal J, Brand M, vanEeden FJM et al. Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. Development. 1996;123:81-93.
Mullins, M C ; Hammerschmidt, M ; Kane, D A ; Odenthal, J ; Brand, M ; vanEeden, F J M ; Furutani-Seiki, Makoto ; Granato, M ; Haffter, P ; Heisenberg, C P ; Jiang, Y J ; Kelsh, R N ; NussleinVolhard, C. / Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. In: Development. 1996 ; Vol. 123. pp. 81-93
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abstract = "We identified 6 genes that are essential for specifying ventral regions of the early zebrafish embryo, Mutations in these genes cause an expansion of structures normally derived from dorsal-lateral regions of the blastula at the expense of ventrally derived structures, A series of phenotypes of varied strengths is observed with different alleles of these mutants, The weakest phenotype is a reduction in the ventral tail fin, observed as a dominant phenotype of swirl, piggytail, and somitabun and a recessive phenotype of min fin, lost-a-fin and some piggytail alleles, With increasing phenotypic strength, the blood and pronephric anlagen are also reduced or absent, while the paraxial mesoderm and anterior neuroectoderm is progressively expanded, In the strong phenotypes, displayed by homozygous embryos of snailhouse, swirl and somitabun, the somites circle around the embryo and the midbrain region is expanded laterally, Several mutations in this group of genes are semidominant as well as recessive indicating a strong dosage sensitivity of the processes involved, Mutations in the piggytail gene display an unusual dominance that depends on both a maternal and zygotic heterozygous genotype, while somitabun is a fully penetrant dominant maternal-effect mutation, The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages, This pathway provides ventral positional information, counteracting the dorsalizing instructions of the organizer, which is localized in the dorsal shield.",
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T1 - Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes

AU - Mullins,M C

AU - Hammerschmidt,M

AU - Kane,D A

AU - Odenthal,J

AU - Brand,M

AU - vanEeden,F J M

AU - Furutani-Seiki,Makoto

AU - Granato,M

AU - Haffter,P

AU - Heisenberg,C P

AU - Jiang,Y J

AU - Kelsh,R N

AU - NussleinVolhard,C

PY - 1996

Y1 - 1996

N2 - We identified 6 genes that are essential for specifying ventral regions of the early zebrafish embryo, Mutations in these genes cause an expansion of structures normally derived from dorsal-lateral regions of the blastula at the expense of ventrally derived structures, A series of phenotypes of varied strengths is observed with different alleles of these mutants, The weakest phenotype is a reduction in the ventral tail fin, observed as a dominant phenotype of swirl, piggytail, and somitabun and a recessive phenotype of min fin, lost-a-fin and some piggytail alleles, With increasing phenotypic strength, the blood and pronephric anlagen are also reduced or absent, while the paraxial mesoderm and anterior neuroectoderm is progressively expanded, In the strong phenotypes, displayed by homozygous embryos of snailhouse, swirl and somitabun, the somites circle around the embryo and the midbrain region is expanded laterally, Several mutations in this group of genes are semidominant as well as recessive indicating a strong dosage sensitivity of the processes involved, Mutations in the piggytail gene display an unusual dominance that depends on both a maternal and zygotic heterozygous genotype, while somitabun is a fully penetrant dominant maternal-effect mutation, The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages, This pathway provides ventral positional information, counteracting the dorsalizing instructions of the organizer, which is localized in the dorsal shield.

AB - We identified 6 genes that are essential for specifying ventral regions of the early zebrafish embryo, Mutations in these genes cause an expansion of structures normally derived from dorsal-lateral regions of the blastula at the expense of ventrally derived structures, A series of phenotypes of varied strengths is observed with different alleles of these mutants, The weakest phenotype is a reduction in the ventral tail fin, observed as a dominant phenotype of swirl, piggytail, and somitabun and a recessive phenotype of min fin, lost-a-fin and some piggytail alleles, With increasing phenotypic strength, the blood and pronephric anlagen are also reduced or absent, while the paraxial mesoderm and anterior neuroectoderm is progressively expanded, In the strong phenotypes, displayed by homozygous embryos of snailhouse, swirl and somitabun, the somites circle around the embryo and the midbrain region is expanded laterally, Several mutations in this group of genes are semidominant as well as recessive indicating a strong dosage sensitivity of the processes involved, Mutations in the piggytail gene display an unusual dominance that depends on both a maternal and zygotic heterozygous genotype, while somitabun is a fully penetrant dominant maternal-effect mutation, The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages, This pathway provides ventral positional information, counteracting the dorsalizing instructions of the organizer, which is localized in the dorsal shield.

KW - maternal effect

KW - tailbud

KW - pattern

KW - gastrulation

KW - dorsoventral axis

KW - zebrafish

KW - dorsalized mutant

KW - ventral determination

KW - formation

M3 - Article

VL - 123

SP - 81

EP - 93

JO - Development

T2 - Development

JF - Development

SN - 0950-1991

ER -