Generation of transgenic medaka expressing claudin7-EGFP for imaging of tight junctions in living medaka embryos

T Miyamoto, A Momoi, K Kato, H Kondoh, S Tsukita, M Furuse, Makoto Furutani-Seiki

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The tight junction (TJ) is a specialized cell-cell adhesion structure in epithelial and endothelial sheets unique to the chordates and functions as a barrier of fluidal diffusion across the cell sheets. In order to study the dynamics of TJ formation in vivo during embryogenesis, we have generated a transgenic medaka line that expresses claudin-7 protein fused to enhanced green fluorescent protein under the regulation of the red seabream beta-actin promoter in transparent medaka embryos. Claudins contain four transmembrane domains and have been identified as the key molecules that dictate the function of TJs. This transgenic medaka line will thus be useful for imaging of TJs in living embryos and hence in screening for mutations affecting cell-cell adhesion.
Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalCell and Tissue Research
Volume335
Issue number2
Early online date27 Nov 2008
DOIs
Publication statusPublished - 1 Feb 2009

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