Abstract
Pancreatic ductal adenocarcinoma (PDAC) is commonly diagnosed at a late stage and becomes resistant to several treatments. Significant clinical effects have been reported for cancer immunotherapies on a subset of patients diagnosed with epithelial cancers. Cancer organoid co-culture with autologous peripheral blood lymphocytes offers an innovative immunotherapeutic approach that is increasingly being tested, although there is a lack of cutting-edge platforms enabling the investigation of cancer-T cell interactions for individual patients. In this study, a pancreatic cancer organoid culture from a genetically engineered pancreatic cancer murine model was established and co-cultured with autologous peripheral blood lymphocytes to induce a tumour-specific T cell response to pancreatic cancer. Co-culturing autologous peripheral blood lymphocytes with cancer organoids can be an effective strategy to enrich tumour-reactive T cells from the peripheral blood of murine models; this approach could potentially be transferred to humans. Co-culture of peripheral blood lymphocytes and cancer organoids could provide an unbiased approach to evaluating the sensitivity of tumour cells to T cell-mediated priming on an individual patient level.
Original language | English |
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Article number | 101365 |
Journal | Biochemistry and Biophysics Reports |
Volume | 32 |
Early online date | 9 Oct 2022 |
DOIs | |
Publication status | Published - 31 Dec 2022 |
Bibliographical note
Funding Information:We thank Dr Chiara Cattaneo from Professor Voest's group at the Department of Molecular Oncology (Netherlands Cancer Institute) for her valuable assistance and advice on the co-culture protocol.
Funding Information:
We thank Oxford Nanopore Technologies and University of Bath for co-funding ADA’s PhD studentship. MFS was supported by the Kakenhi grant 19K22661 from MEXT and Tokuteikenkyu grant from the Takeda Science Foundation .
Funding
We thank Dr Chiara Cattaneo from Professor Voest's group at the Department of Molecular Oncology (Netherlands Cancer Institute) for her valuable assistance and advice on the co-culture protocol. We thank Oxford Nanopore Technologies and University of Bath for co-funding ADA’s PhD studentship. MFS was supported by the Kakenhi grant 19K22661 from MEXT and Tokuteikenkyu grant from the Takeda Science Foundation .
Keywords
- Cancer organoids
- Co-culture
- Murine model
- Pancreatic cancer
- Peripheral blood mononuclear cell
- Tumour microenvironment
ASJC Scopus subject areas
- Biophysics
- Biochemistry