Generation of mice with functional inactivation of talpid3, a gene first identified in chicken

Fiona Bangs, Nicole Antonio, P Thongnuek, Monique Welten, M G Davey, J Briscoe, Cheryll Tickle

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Specification of digit number and identity is central to digit pattern in vertebrate limbs. The classical talpid(3) chicken mutant has many unpatterned digits together with defects in other regions, depending on hedgehog (Hh) signalling, and exhibits embryonic lethality. The talpid(3) chicken has a mutation in KIAA0586, which encodes a centrosomal protein required for the formation of primary cilia, which are sites of vertebrate Hh signalling. The highly conserved exons 11 and 12 of KIAA0586 are essential to rescue cilia in talpid(3) chicken mutants. We constitutively deleted these two exons to make a talpid(3-/-) mouse. Mutant mouse embryos lack primary cilia and, like talpid(3) chicken embryos, have face and neural tube defects but also defects in left/right asymmetry. Conditional deletion in mouse limb mesenchyme results in polydactyly and in brachydactyly and a failure of subperisoteal bone formation, defects that are attributable to abnormal sonic hedgehog and Indian hedgehog signalling, respectively. Like talpid(3) chicken limbs, the mutant mouse limbs are syndactylous with uneven digit spacing as reflected in altered Raldh2 expression, which is normally associated with interdigital mesenchyme. Both mouse and chicken mutant limb buds are broad and short. talpid(3-/-) mouse cells migrate more slowly than wild-type mouse cells, a change in cell behaviour that possibly contributes to altered limb bud morphogenesis. This genetic mouse model will facilitate further conditional approaches, epistatic experiments and open up investigation into the function of the novel talpid3 gene using the many resources available for mice.
Original languageEnglish
Pages (from-to)3261-3272
Number of pages12
JournalDevelopment
Volume138
Issue number15
DOIs
Publication statusPublished - 1 Aug 2011

Fingerprint

Chickens
Hedgehogs
Genes
Cilia
Extremities
Limb Buds
Mesoderm
Vertebrates
Exons
Embryonic Structures
Brachydactyly
Polydactyly
Neural Tube Defects
Genetic Models
Morphogenesis
Osteogenesis
Mutation
Proteins

Keywords

  • primary cilia
  • talpid3
  • mouse mutant
  • hedgehog signalling
  • polydactyly
  • limb

Cite this

Bangs, F., Antonio, N., Thongnuek, P., Welten, M., Davey, M. G., Briscoe, J., & Tickle, C. (2011). Generation of mice with functional inactivation of talpid3, a gene first identified in chicken. Development, 138(15), 3261-3272. https://doi.org/10.1242/dev.063602

Generation of mice with functional inactivation of talpid3, a gene first identified in chicken. / Bangs, Fiona; Antonio, Nicole; Thongnuek, P; Welten, Monique; Davey, M G; Briscoe, J; Tickle, Cheryll.

In: Development, Vol. 138, No. 15, 01.08.2011, p. 3261-3272.

Research output: Contribution to journalArticle

Bangs, F, Antonio, N, Thongnuek, P, Welten, M, Davey, MG, Briscoe, J & Tickle, C 2011, 'Generation of mice with functional inactivation of talpid3, a gene first identified in chicken', Development, vol. 138, no. 15, pp. 3261-3272. https://doi.org/10.1242/dev.063602
Bangs F, Antonio N, Thongnuek P, Welten M, Davey MG, Briscoe J et al. Generation of mice with functional inactivation of talpid3, a gene first identified in chicken. Development. 2011 Aug 1;138(15):3261-3272. https://doi.org/10.1242/dev.063602
Bangs, Fiona ; Antonio, Nicole ; Thongnuek, P ; Welten, Monique ; Davey, M G ; Briscoe, J ; Tickle, Cheryll. / Generation of mice with functional inactivation of talpid3, a gene first identified in chicken. In: Development. 2011 ; Vol. 138, No. 15. pp. 3261-3272.
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