Generation of medaka gene knockout models by target-selected mutagenesis

Yoshihito Taniguchi, Shunichi Takeda, Makoto Furutani-Seiki, Yasuhiro Kamei, Takeshi Todo, Takao Sasado, Tomonori Deguchi, Hisato Kondoh, Josine Mudde, Mitsuyoshi Yamazoe, Masayuki Hidaka, Hiroshi Mitani, Atsushi Toyoda, Yoshiyuki Sakaki, Ronald HA Plasterk, Edwin Cuppen

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)

Abstract

We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.
Original languageEnglish
Article numberR116
JournalGenome Biology
Volume7
Issue number12
DOIs
Publication statusPublished - 2006

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