Generation of medaka gene knockout models by target-selected mutagenesis

Yoshihito Taniguchi; Shunichi Takeda; Makoto Furutani-Seiki; Yasuhiro Kamei; Takeshi Todo; Takao Sasado; Tomonori Deguchi; Hisato Kondoh; Josine Mudde; Mitsuyoshi Yamazoe; Masayuki Hidaka; Hiroshi Mitani; Atsushi Toyoda; Yoshiyuki Sakaki; Ronald HA Plasterk; Edwin Cuppen

doi:10.1186/gb-2006-7-12-r116

Generation of medaka gene knockout models by target-selected mutagenesis

Yoshihito Taniguchi, Shunichi Takeda, Makoto Furutani-Seiki, Yasuhiro Kamei, Takeshi Todo, Takao Sasado, Tomonori Deguchi, Hisato Kondoh, Josine Mudde, Mitsuyoshi Yamazoe, Masayuki Hidaka, Hiroshi Mitani, Atsushi Toyoda, Yoshiyuki Sakaki, Ronald HA Plasterk, Edwin Cuppen

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

124 Citations (SciVal)

Abstract

We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

Original language	English
Article number	R116
Journal	Genome Biology
Volume	7
Issue number	12
DOIs	https://doi.org/10.1186/gb-2006-7-12-r116
Publication status	Published - 2006

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10.1186/gb-2006-7-12-r116

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title = "Generation of medaka gene knockout models by target-selected mutagenesis",

abstract = "We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.",

author = "Yoshihito Taniguchi and Shunichi Takeda and Makoto Furutani-Seiki and Yasuhiro Kamei and Takeshi Todo and Takao Sasado and Tomonori Deguchi and Hisato Kondoh and Josine Mudde and Mitsuyoshi Yamazoe and Masayuki Hidaka and Hiroshi Mitani and Atsushi Toyoda and Yoshiyuki Sakaki and Plasterk, {Ronald HA} and Edwin Cuppen",

year = "2006",

doi = "10.1186/gb-2006-7-12-r116",

language = "English",

volume = "7",

journal = "Genome Biology",

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TY - JOUR

T1 - Generation of medaka gene knockout models by target-selected mutagenesis

AU - Taniguchi, Yoshihito

AU - Takeda, Shunichi

AU - Furutani-Seiki, Makoto

AU - Kamei, Yasuhiro

AU - Todo, Takeshi

AU - Sasado, Takao

AU - Deguchi, Tomonori

AU - Kondoh, Hisato

AU - Mudde, Josine

AU - Yamazoe, Mitsuyoshi

AU - Hidaka, Masayuki

AU - Mitani, Hiroshi

AU - Toyoda, Atsushi

AU - Sakaki, Yoshiyuki

AU - Plasterk, Ronald HA

AU - Cuppen, Edwin

PY - 2006

Y1 - 2006

N2 - We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

AB - We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

UR - http://dx.doi.org/10.1186/gb-2006-7-12-r116

U2 - 10.1186/gb-2006-7-12-r116

DO - 10.1186/gb-2006-7-12-r116

M3 - Article

VL - 7

JO - Genome Biology

JF - Genome Biology

IS - 12

M1 - R116

ER -

Generation of medaka gene knockout models by target-selected mutagenesis

Abstract

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