Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling

Emily Lay; Tressan Grant; Gernot Walko; Ute Jungwirth

doi:10.1007/978-1-0716-3834-7_2

Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling

Emily Lay, Tressan Grant, Gernot Walko, Ute Jungwirth

Research output: Chapter or section in a book/report/conference proceeding › Book chapter

Abstract

Fibroblasts are the major producers of the extracellular matrix and regulate its organization. Aberrant signaling in diseases such as fibrosis and cancer can impact the deposition of the matrix proteins, which can in turn act as an adhesion scaffold and signaling reservoir promoting disease progression. To study the composition and organization of the extracellular matrix as well as its interactions with (tumor) cells, this protocol describes the generation and analysis of 3D fibroblast-derived matrices and the investigation of (tumor) cells seeded onto the 3D scaffolds by immunofluorescent imaging and cell adhesion, colony formation, migration, and invasion/transmigration assays.

Original language	English
Title of host publication	Imaging Cell Signaling
Editors	C. Wuelfing, R. F. Murphy
Place of Publication	New York, U. S. A.
Publisher	Humana Press
Chapter	2
Pages	11-25
Number of pages	15
Volume	2800
Edition	1st
ISBN (Electronic)	9781071638347
ISBN (Print)	9781071638330
DOIs	https://doi.org/10.1007/978-1-0716-3834-7_2
Publication status	Published - 7 May 2024

Publication series

Name	Methods in Molecular Biology
Volume	2800
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

This work was supported in parts by grant 016/S/20 from the British Skin Foundation awarded to Ute Jungwirth, by grant BB/T012978/1 from the Biotechnology and Biological Sciences Research Council BBSRC/UKRI awarded to Gernot Walko, and by grant MR/W006308/1 for the GW4 BIOMED2 DTP awarded to the Universities of Bath, Bristol, Cardiff and Exeter from the Medical Research Council (MRC)/UKRI. Figure 1 was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license.

Funders	Funder number
Medical Research Council
Biotechnology and Biological Sciences Research Council
Servier
British Skin Foundation	BB/T012978/1
British Skin Foundation
UK Research & Innovation	MR/W006308/1
UK Research & Innovation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell-derived matrix
Cell-matrix interaction
Extracellular matrix
Fibroblasts
Fibronectin
Matrix adhesion
Matrix deposition assay

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-3834-7_2

Cite this

Lay, E., Grant, T., Walko, G., & Jungwirth, U. (2024). Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling. In C. Wuelfing, & R. F. Murphy (Eds.), Imaging Cell Signaling (1st ed., Vol. 2800, pp. 11-25). (Methods in Molecular Biology ; Vol. 2800). Humana Press. https://doi.org/10.1007/978-1-0716-3834-7_2

Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling. / Lay, Emily; Grant, Tressan ; Walko, Gernot et al.
Imaging Cell Signaling. ed. / C. Wuelfing; R. F. Murphy. Vol. 2800 1st. ed. New York, U. S. A.: Humana Press, 2024. p. 11-25 (Methods in Molecular Biology ; Vol. 2800).

Research output: Chapter or section in a book/report/conference proceeding › Book chapter

Lay, E, Grant, T , Walko, G & Jungwirth, U 2024, Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling. in C Wuelfing & RF Murphy (eds), Imaging Cell Signaling. 1st edn, vol. 2800, Methods in Molecular Biology , vol. 2800, Humana Press, New York, U. S. A., pp. 11-25. https://doi.org/10.1007/978-1-0716-3834-7_2

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Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling

Abstract

Publication series

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

The transcriptional control of human epidermal stemness.

Targeting YAP/TAZ as a novel therapeutic strategy in cutaneous squamous cell carcinoma.

Cite this

Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling

Abstract

Publication series

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

The transcriptional control of human epidermal stemness.

Targeting YAP/TAZ as a novel therapeutic strategy in cutaneous squamous cell carcinoma.

Cite this