Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Evangelos Mourkas, Diego Florez-Cuadrado, Ben Pascoe, Jessica K Calland, Sion C Bayliss, Leonardos Mageiros, Guillaume Méric, Matthew D Hitchings, Alberto Quesada, Concepción Porrero, María Ugarte-Ruiz, José Gutiérrez-Fernández, Lucas Domínguez, Samuel K Sheppard

Research output: Contribution to journalArticle

Abstract

The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. Antimicrobial resistance was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands, often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalEnvironmental Microbiology
Early online date5 Aug 2019
DOIs
Publication statusE-pub ahead of print - 5 Aug 2019

Cite this

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease. / Mourkas, Evangelos; Florez-Cuadrado, Diego; Pascoe, Ben; Calland, Jessica K; Bayliss, Sion C; Mageiros, Leonardos; Méric, Guillaume; Hitchings, Matthew D; Quesada, Alberto; Porrero, Concepción; Ugarte-Ruiz, María; Gutiérrez-Fernández, José; Domínguez, Lucas; Sheppard, Samuel K.

In: Environmental Microbiology, 05.08.2019.

Research output: Contribution to journalArticle

Mourkas, Evangelos ; Florez-Cuadrado, Diego ; Pascoe, Ben ; Calland, Jessica K ; Bayliss, Sion C ; Mageiros, Leonardos ; Méric, Guillaume ; Hitchings, Matthew D ; Quesada, Alberto ; Porrero, Concepción ; Ugarte-Ruiz, María ; Gutiérrez-Fernández, José ; Domínguez, Lucas ; Sheppard, Samuel K. / Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease. In: Environmental Microbiology. 2019.
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AU - Mourkas, Evangelos

AU - Florez-Cuadrado, Diego

AU - Pascoe, Ben

AU - Calland, Jessica K

AU - Bayliss, Sion C

AU - Mageiros, Leonardos

AU - Méric, Guillaume

AU - Hitchings, Matthew D

AU - Quesada, Alberto

AU - Porrero, Concepción

AU - Ugarte-Ruiz, María

AU - Gutiérrez-Fernández, José

AU - Domínguez, Lucas

AU - Sheppard, Samuel K

N1 - This article is protected by copyright. All rights reserved.

PY - 2019/8/5

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N2 - The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. Antimicrobial resistance was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands, often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment. This article is protected by copyright. All rights reserved.

AB - The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. Antimicrobial resistance was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands, often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment. This article is protected by copyright. All rights reserved.

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DO - 10.1111/1462-2920.14760

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