Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Evangelos Mourkas; Diego Florez-Cuadrado; Ben Pascoe; Jessica K Calland; Sion C Bayliss; Leonardos Mageiros; Guillaume Méric; Matthew D Hitchings; Alberto Quesada; Concepción Porrero; María Ugarte-Ruiz; José Gutiérrez-Fernández; Lucas Domínguez; Samuel K Sheppard

doi:10.1111/1462-2920.14760

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Evangelos Mourkas, Diego Florez-Cuadrado, Ben Pascoe, Jessica K Calland, Sion C Bayliss, Leonardos Mageiros, Guillaume Méric, Matthew D Hitchings, Alberto Quesada, Concepción Porrero, María Ugarte-Ruiz, José Gutiérrez-Fernández, Lucas Domínguez, Samuel K Sheppard

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Abstract

The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment.

Original language	English
Pages (from-to)	4597-4613
Number of pages	17
Journal	Environmental Microbiology
Volume	21
Issue number	12
Early online date	5 Aug 2019
DOIs	https://doi.org/10.1111/1462-2920.14760
Publication status	Published - 31 Dec 2019

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1111/1462-2920.14760Licence: CC BY

2019 07 Mourkas.DiegoFC.etal_CampySewageAMR_EM_accepted.PDF
This is the peer reviewed version of the following article: Mourkas, E. , Florez‐Cuadrado, D. , Pascoe, B. , Calland, J. K., Bayliss, S. C., Mageiros, L. , Méric, G. , Hitchings, M. D., Quesada, A. , Porrero, C. , Ugarte‐Ruiz, M. , Gutiérrez‐Fernández, J. , Domínguez, L. and Sheppard, S. K. (2019), Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease. Environ Microbiol. Accepted Author Manuscript, which has been published in final form at https://doi.org/10.1111/1462-2920.14760. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Accepted author manuscript, 3.3 MBLicence: Unspecified

Cite this

Mourkas, E., Florez-Cuadrado, D., Pascoe, B., Calland, J. K., Bayliss, S. C., Mageiros, L., Méric, G., Hitchings, M. D., Quesada, A., Porrero, C., Ugarte-Ruiz, M., Gutiérrez-Fernández, J., Domínguez, L., & Sheppard, S. K. (2019). Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease. Environmental Microbiology, 21(12), 4597-4613. https://doi.org/10.1111/1462-2920.14760

Mourkas, E, Florez-Cuadrado, D, Pascoe, B, Calland, JK, Bayliss, SC, Mageiros, L, Méric, G, Hitchings, MD, Quesada, A, Porrero, C, Ugarte-Ruiz, M, Gutiérrez-Fernández, J, Domínguez, L & Sheppard, SK 2019, 'Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease', Environmental Microbiology, vol. 21, no. 12, pp. 4597-4613. https://doi.org/10.1111/1462-2920.14760

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abstract = "The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment.",

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AU - Hitchings, Matthew D

AU - Quesada, Alberto

AU - Porrero, Concepción

AU - Ugarte-Ruiz, María

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Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Abstract

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