Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells

Joseph D Dukes, Laura Fish, Judith D Richardson, Elizabeth Blaikley, Samir Burns, Christopher J Caunt, Andrew D Chalmers, Paul Whitley

Research output: Contribution to journalArticle

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Abstract

Genetic screens in Drosophila have identified regulators of endocytic trafficking as neoplastic tumor suppressor genes. For example, Drosophila endosomal sorting complex required for transport (ESCRT) mutants lose epithelial polarity and show increased cell proliferation, suggesting that ESCRT proteins could function as tumor suppressors. In this study, we show for the for the first time to our knowledge that ESCRT proteins are required to maintain polarity in mammalian epithelial cells. Inhibition of ESCRT function caused the tight junction protein claudin-1 to accumulate in intracellular vesicles. In contrast E-cadherin and occludin localization was unaffected. We investigated the cause of this accumulation and show that claudin-1 is constitutively recycled in kidney, colon, and lung epithelial cells, identifying claudin-1 recycling as a newly described feature of diverse epithelial cell types. This recycling requires ESCRT function, explaining the accumulation of intracellular claudin-1 when ESCRT function is inhibited. We further demonstrate that small interfering RNA knockdown of the ESCRT protein Tsg101 causes epithelial monolayers to lose their polarized organization and interferes with the establishment of a normal epithelial permeability barrier. ESCRT knockdown also reduces the formation of correctly polarized three-dimensional cysts. Thus, in mammalian epithelial cells, ESCRT function is required for claudin-1 trafficking and for epithelial cell polarity, supporting the hypothesis that ESCRT proteins function as tumor suppressors.
LanguageEnglish
Pages3192-3205
Number of pages14
JournalMolecular Biology of the Cell
Volume22
Issue number17
Early online date13 Jul 2011
DOIs
StatusPublished - 1 Sep 2011

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Endosomal Sorting Complexes Required for Transport
Claudin-1
Recycling
Vertebrates
Epithelial Cells
Maintenance
Carrier Proteins
Drosophila
Zonula Occludens-1 Protein
Occludin
Cell Polarity
Cadherins
Tumor Suppressor Genes
Small Interfering RNA
Cysts
Permeability
Neoplasms
Colon

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Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells. / Dukes, Joseph D; Fish, Laura; Richardson, Judith D; Blaikley, Elizabeth; Burns, Samir; Caunt, Christopher J; Chalmers, Andrew D; Whitley, Paul.

In: Molecular Biology of the Cell, Vol. 22, No. 17, 01.09.2011, p. 3192-3205.

Research output: Contribution to journalArticle

Dukes, Joseph D ; Fish, Laura ; Richardson, Judith D ; Blaikley, Elizabeth ; Burns, Samir ; Caunt, Christopher J ; Chalmers, Andrew D ; Whitley, Paul. / Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 17. pp. 3192-3205
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