Functional and Genetic Analysis of Plectin in Skin and Muscle

Günther A. Rezniczek, Lilli Winter, Gernot Walko, Gerhard Wiche

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Plectin is a large cytoskeletal linker protein with a multitude of functions affecting various cellular processes. It is expressed as several different isoforms from a highly complex gene. Both, this transcript diversity (mainly caused by short 5′-sequences contained in alternative first exons) and the size (> 500 kDa) of the resulting proteins, present considerable challenges to plectin researchers. In this chapter, we will consider these problems and offer advice on how to tackle them best. As plectin has been studied most extensively in skin and muscle, we will focus on these types of tissues and describe some selected methods in detail. Foremost, however, we aim to give the readers some good pointers to available tools and into the existing literature.
Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherElsevier Academic Press Inc
Pages235-259
Number of pages25
Volume569
DOIs
Publication statusPublished - 2016

Keywords

  • Cytoarchitecture
  • Cytolinker proteins
  • Plakins
  • Plectin
  • Protein isoforms
  • Signaling scaffold

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    Rezniczek, G. A., Winter, L., Walko, G., & Wiche, G. (2016). Functional and Genetic Analysis of Plectin in Skin and Muscle. In Methods in Enzymology (Vol. 569, pp. 235-259). Elsevier Academic Press Inc. https://doi.org/10.1016/bs.mie.2015.05.003