Functional analysis of the secretory precursor processing machinery of Bacillus subtilis: Identification of a eubacterial homolog of archaeal and eukaryotic signal peptidases

Harold Tjalsma, Albert Bolhuis, Maarten L. Van Roosmalen, Thomas Wiegert, Wolfgang Schumann, Cees P. Broekhuizen, Wim J. Quax, Gerard Venema, Sierd Bron, Jan Maarten Van Dijl

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

Approximately 47% of the genes of the Gram-positive bacterium Bacillus subtilis belong to paralogous gene families. The present studies were aimed at the functional analysis of the sip gene family of B. subtilis, consisting of five chromosomal genes, denoted sipS, sipT, sipU, sipV, and sipW. All five sip genes specify type I signal peptidases (SPases), which are actively involved in the processing of secretory preproteins. Interestingly, strains lacking as many as four of these SPases could be obtained. As shown with a temperature-sensitive SipS variant, only cells lacking both SipS and SipT were not viable, which may be caused by jamming of the secretion machinery with secretory preproteins. Thus, SipS and SipT are of major importance for protein secretion. This conclusion is underscored by the observation that only the transcription of the sips and sipT genes is temporally controlled via the DegS-DegU regulatory system, in concert with the transcription of most genes for secretory preproteins. Notably, the newly identified SPase SipW is highly similar to SPases from archaea and the ER membrane of eukaryotes, suggesting that these enzymes form a subfamily of the type I SPases, which is conserved in the three domains of life.

Original languageEnglish
Pages (from-to)2318-2331
Number of pages14
JournalGenes and Development
Volume12
Issue number15
DOIs
Publication statusPublished - 1 Aug 1998

Keywords

  • Bacillus subtilis
  • Leader peptidase
  • Paralogous gene family
  • Protein secretion
  • Signal peptidase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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