Full-wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe; Kishan Dholakia; Peter R.T. Munro

doi:10.1109/ipc53466.2022.9975671

Full-wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe, Kishan Dholakia, Peter R.T. Munro

Department of Physics

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Abstract

Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

Original language	English
Title of host publication	2022 IEEE photonics conference (IPC)
DOIs	https://doi.org/10.1109/ipc53466.2022.9975671
Publication status	Published - 14 Dec 2022

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10.1109/ipc53466.2022.9975671

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abstract = "Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.",

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AU - Munro, Peter R.T.

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N2 - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

AB - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

UR - https://research-portal.st-andrews.ac.uk/en/publications/6bb08f7b-a831-4eac-934a-3592a6d270f7

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DO - 10.1109/ipc53466.2022.9975671

M3 - Chapter in a published conference proceeding

SN - 9781665434874

SN - 9781665434881

BT - 2022 IEEE photonics conference (IPC)

ER -

Full-wave modelling of two-photon microscopy with spatiotemporal focussing

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