Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei

T Wakayama, Anthony C F Perry, M Zuccotti, K R Johnson, R Yanagimachi

Research output: Contribution to journalArticle

1809 Citations (Scopus)

Abstract

Until recently, fertilization was the only way to produce viable mammalian offspring, a process implicitly involving male and female gametes. However, techniques involving fusion of embryonic or fetal somatic cells with enucleated oocytes have become steadily more successful in generating cloned young. Dolly the sheep was produced by electrofusion of sheep mammary-derived cells with enucleated sheep oocytes. Here we investigate the factors governing embryonic development by introducing nuclei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mouse oocytes. We found that some enucleated oocytes receiving Sertoli or neuronal nuclei developed in vitro and implanted following transfer, but none developed beyond 8.5 days post coitum; however, a high percentage of enucleated oocytes receiving cumulus nuclei developed in vitro. Once transferred, many of these embryos implanted and, although most were subsequently resorbed, a significant proportion (2 to 2.8%) developed to term. These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
LanguageEnglish
Pages369-374
Number of pages6
JournalNature
Volume394
Issue number6691
DOIs
StatusPublished - 1998

Fingerprint

Cumulus Cells
Cell Nucleus
Oocytes
Sheep
Sertoli Cells
Fertilization
Germ Cells
Embryonic Development
Mammals
Breast
Embryonic Structures
Phenotype

Cite this

Wakayama, T., Perry, A. C. F., Zuccotti, M., Johnson, K. R., & Yanagimachi, R. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394(6691), 369-374. https://doi.org/10.1038/28615

Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. / Wakayama, T; Perry, Anthony C F; Zuccotti, M; Johnson, K R; Yanagimachi, R.

In: Nature, Vol. 394, No. 6691, 1998, p. 369-374.

Research output: Contribution to journalArticle

Wakayama, T, Perry, ACF, Zuccotti, M, Johnson, KR & Yanagimachi, R 1998, 'Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei', Nature, vol. 394, no. 6691, pp. 369-374. https://doi.org/10.1038/28615
Wakayama, T ; Perry, Anthony C F ; Zuccotti, M ; Johnson, K R ; Yanagimachi, R. / Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. In: Nature. 1998 ; Vol. 394, No. 6691. pp. 369-374.
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