Frequency of autoantibodies to a major epitope on the carboxyl terminal fragment of CENP-B in patients with autoimmune disease

J Whyte, E Soriano, W C Earnshaw, N J McHugh

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The carboxyl-terminal fragment of CENP-B contains a major epitope for anti-centromere antibodies (ACA). We have developed an enzyme-linked immunoassay (ELISA) for measuring antibodies to the 147-carboxyl-terminal amino acids of CENP-B expressed as a beta-galactosidase fusion protein. The ELISA was 98% sensitive and 95% specific for detecting ACA in a population which included 46 patients with ACA detected by other means. Therefore, the CENP-B ELISA should prove a valuable tool in screening for ACA in populations at risk of developing systemic sclerosis, such as those with Raynaud's phenomenon. Levels of anti-CENP-B antibodies were not increased in unaffected relatives of probands with ACA.
Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalBritish Journal of Rheumatology
Issue number5
Publication statusPublished - 5 May 1995


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