Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules

Kai Bin Li, Na Li, Yi Zang, Guo Rong Chen, Jia Li, Tony D. James, Xiao Peng He, He Tian

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Small-molecular probes capable of monitoring and interfering with the activity of biomacromolecules-such as polysaccharides, nucleotides and proteins-are of paramount importance to the advancement of life science. However, such probes that can detect and simultaneously modulate the construction of biomacromolecules are elusive. Here we report a fluorogenic, foldable glycoprobe that can recognize and assemble a protein receptor in a synchronous fashion. The glycoprobe synthesized by introducing a glycoligand (for protein recognition) to a bola-type bis-fluorophore conjugate shows a "self-shielded" fluorescence in the folded state. Association with a receptor protein rapidly unfolds the probe, releasing a fluorophore capable of crosslinking the proteins-as determined using small-angle X-ray scattering-thereby producing a unique fluorescent supramolecular construct. We have demonstrated the use of the foldable glycoprobe in order to track the endocytic cycle of a transmembrane receptor.

Original languageEnglish
Pages (from-to)6325-6329
Number of pages5
JournalChemical Science
Volume7
Issue number10
DOIs
Publication statusPublished - 2016

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Crosslinking
Fluorophores
Proteins
Molecular Probes
X ray scattering
Polysaccharides
Nucleotides
Fluorescence
Monitoring

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Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules. / Li, Kai Bin; Li, Na; Zang, Yi; Chen, Guo Rong; Li, Jia; James, Tony D.; He, Xiao Peng; Tian, He.

In: Chemical Science, Vol. 7, No. 10, 2016, p. 6325-6329.

Research output: Contribution to journalArticle

Li, KB, Li, N, Zang, Y, Chen, GR, Li, J, James, TD, He, XP & Tian, H 2016, 'Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules', Chemical Science, vol. 7, no. 10, pp. 6325-6329. https://doi.org/10.1039/c6sc02366e
Li, Kai Bin ; Li, Na ; Zang, Yi ; Chen, Guo Rong ; Li, Jia ; James, Tony D. ; He, Xiao Peng ; Tian, He. / Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules. In: Chemical Science. 2016 ; Vol. 7, No. 10. pp. 6325-6329.
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