Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules

Kai Bin Li, Na Li, Yi Zang, Guo Rong Chen, Jia Li, Tony D. James, Xiao Peng He, He Tian

Research output: Contribution to journalArticlepeer-review

31 Citations (SciVal)


Small-molecular probes capable of monitoring and interfering with the activity of biomacromolecules-such as polysaccharides, nucleotides and proteins-are of paramount importance to the advancement of life science. However, such probes that can detect and simultaneously modulate the construction of biomacromolecules are elusive. Here we report a fluorogenic, foldable glycoprobe that can recognize and assemble a protein receptor in a synchronous fashion. The glycoprobe synthesized by introducing a glycoligand (for protein recognition) to a bola-type bis-fluorophore conjugate shows a "self-shielded" fluorescence in the folded state. Association with a receptor protein rapidly unfolds the probe, releasing a fluorophore capable of crosslinking the proteins-as determined using small-angle X-ray scattering-thereby producing a unique fluorescent supramolecular construct. We have demonstrated the use of the foldable glycoprobe in order to track the endocytic cycle of a transmembrane receptor.

Original languageEnglish
Pages (from-to)6325-6329
Number of pages5
JournalChemical Science
Issue number10
Publication statusPublished - 2016


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