Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules

Kai Bin Li, Na Li, Yi Zang, Guo Rong Chen, Jia Li, Tony D. James, Xiao Peng He, He Tian

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Small-molecular probes capable of monitoring and interfering with the activity of biomacromolecules-such as polysaccharides, nucleotides and proteins-are of paramount importance to the advancement of life science. However, such probes that can detect and simultaneously modulate the construction of biomacromolecules are elusive. Here we report a fluorogenic, foldable glycoprobe that can recognize and assemble a protein receptor in a synchronous fashion. The glycoprobe synthesized by introducing a glycoligand (for protein recognition) to a bola-type bis-fluorophore conjugate shows a "self-shielded" fluorescence in the folded state. Association with a receptor protein rapidly unfolds the probe, releasing a fluorophore capable of crosslinking the proteins-as determined using small-angle X-ray scattering-thereby producing a unique fluorescent supramolecular construct. We have demonstrated the use of the foldable glycoprobe in order to track the endocytic cycle of a transmembrane receptor.

Original languageEnglish
Pages (from-to)6325-6329
Number of pages5
JournalChemical Science
Volume7
Issue number10
DOIs
Publication statusPublished - 2016

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    Li, K. B., Li, N., Zang, Y., Chen, G. R., Li, J., James, T. D., He, X. P., & Tian, H. (2016). Foldable glycoprobes capable of fluorogenic crosslinking of biomacromolecules. Chemical Science, 7(10), 6325-6329. https://doi.org/10.1039/c6sc02366e