Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

T L Williams; Mmlm Vareiro; A T A Jenkins

doi:10.1021/la060853q

Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

T L Williams, Mmlm Vareiro, A T A Jenkins

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (SciVal)

Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

Original language	English
Pages (from-to)	6473-6476
Number of pages	4
Journal	Langmuir
Volume	22
Issue number	15
DOIs	https://doi.org/10.1021/la060853q
Publication status	Published - 2006

Bibliographical note

ID number: ISI:000238926000004

Access to Document

10.1021/la060853q

Cite this

@article{bdf8ee1ec3cf4bcaaf20954aa8def4b6,

title = "Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes",

abstract = "This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.",

author = "Williams, \{T L\} and Mmlm Vareiro and Jenkins, \{A T A\}",

note = "ID number: ISI:000238926000004",

year = "2006",

doi = "10.1021/la060853q",

language = "English",

volume = "22",

pages = "6473--6476",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "15",

}

TY - JOUR

T1 - Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

AU - Williams, T L

AU - Vareiro, Mmlm

AU - Jenkins, A T A

N1 - ID number: ISI:000238926000004

PY - 2006

Y1 - 2006

N2 - This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

AB - This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

UR - https://www.scopus.com/pages/publications/33746637064

U2 - 10.1021/la060853q

DO - 10.1021/la060853q

M3 - Article

SN - 0743-7463

VL - 22

SP - 6473

EP - 6476

JO - Langmuir

JF - Langmuir

IS - 15

ER -

Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this