Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

T L Williams; Mmlm Vareiro; A T A Jenkins

doi:10.1021/la060853q

Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

T L Williams, Mmlm Vareiro, A T A Jenkins

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (SciVal)

Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

Original language	English
Pages (from-to)	6473-6476
Number of pages	4
Journal	Langmuir
Volume	22
Issue number	15
DOIs	https://doi.org/10.1021/la060853q
Publication status	Published - 2006

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ID number: ISI:000238926000004

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title = "Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes",

abstract = "This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.",

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AU - Williams, T L

AU - Vareiro, Mmlm

AU - Jenkins, A T A

N1 - ID number: ISI:000238926000004

PY - 2006

Y1 - 2006

N2 - This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

AB - This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

U2 - 10.1021/la060853q

DO - 10.1021/la060853q

M3 - Article

SN - 0743-7463

VL - 22

SP - 6473

EP - 6476

JO - Langmuir

JF - Langmuir

IS - 15

ER -

Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

Abstract

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