Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes

T L Williams, Mmlm Vareiro, A T A Jenkins

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.
Original languageEnglish
Pages (from-to)6473-6476
Number of pages4
JournalLangmuir
Volume22
Issue number15
DOIs
Publication statusPublished - 2006

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