Fluorogenic probes for detecting deacylase and demethylase activity towards post-translationally-modified lysine residues

Yuichiro Hori, Miyako Nishiura, Tomomi Tao, Reisuke Baba, Steven D. Bull, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

Abstract

Reversible enzymatic post-translational modification of the ε-amino groups of lysine residues (e.g. N-acylation reactions) plays an important role in regulating the cellular activities of numerous proteins. This study describes how enzyme catalyzed N-deprotection of lysine residues of non-fluorescent peptide-coumarin probes can be used to generate N-deprotected peptides that undergo spontaneousO- toN-ester transfer reactions (uncatalyzed) to generate a highly fluorescentN-carbamoyl peptide. This enables detection of enzyme catalyzedN-deacetylation,N-demalonylation,N-desuccinylation andN-demethylation reactions activities towards the N-modified lysine residues of these probes using simple ‘turn on’ fluorescent assays.

Original languageEnglish
Pages (from-to)2498-2503
Number of pages6
JournalChemical Science
Volume12
Issue number7
Early online date7 Feb 2021
DOIs
Publication statusPublished - 21 Feb 2021

ASJC Scopus subject areas

  • Chemistry(all)

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