Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs

George M. Neville; Karen J. Edler; Gareth J. Price

doi:10.1039/d1nr07230g

Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs

George M. Neville, Karen J. Edler, Gareth J. Price

Research output: Contribution to journal › Article › peer-review

5 Citations (SciVal)

Abstract

Fluorescently-labelled variants of poly(styrene-co-maleic acid), SMA, have been synthesised by RAFT copolymerisation. We show that low ratios of vinyl fluorophores, analogous to styrene, can be successfully incorporated during polymerisation without detriment to nanodisc formation upon interaction with lipids. These novel copolymers are capable of encapuslating lipids and the model membrane protein, gramicidin, and hence have the potential to be applied in fluorescence-based biological studies. To demonstrate this, energy transfer is used to probe polymer-protein interactions in nanodiscs. The copolymers may also be used to monitor nanodisc self assembly by exploiting aggregation-caused-quenching (ACQ).

Original language	English
Pages (from-to)	5689-5693
Number of pages	5
Journal	Nanoscale
Volume	14
Issue number	15
Early online date	17 Mar 2022
DOIs	https://doi.org/10.1039/d1nr07230g
Publication status	Published - 21 Apr 2022

Bibliographical note

Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council EP/L016354/1.

ASJC Scopus subject areas

General Materials Science

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10.1039/d1nr07230gLicence: CC BY

Cite this

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abstract = "Fluorescently-labelled variants of poly(styrene-co-maleic acid), SMA, have been synthesised by RAFT copolymerisation. We show that low ratios of vinyl fluorophores, analogous to styrene, can be successfully incorporated during polymerisation without detriment to nanodisc formation upon interaction with lipids. These novel copolymers are capable of encapuslating lipids and the model membrane protein, gramicidin, and hence have the potential to be applied in fluorescence-based biological studies. To demonstrate this, energy transfer is used to probe polymer-protein interactions in nanodiscs. The copolymers may also be used to monitor nanodisc self assembly by exploiting aggregation-caused-quenching (ACQ).",

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AU - Edler, Karen J.

AU - Price, Gareth J.

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AB - Fluorescently-labelled variants of poly(styrene-co-maleic acid), SMA, have been synthesised by RAFT copolymerisation. We show that low ratios of vinyl fluorophores, analogous to styrene, can be successfully incorporated during polymerisation without detriment to nanodisc formation upon interaction with lipids. These novel copolymers are capable of encapuslating lipids and the model membrane protein, gramicidin, and hence have the potential to be applied in fluorescence-based biological studies. To demonstrate this, energy transfer is used to probe polymer-protein interactions in nanodiscs. The copolymers may also be used to monitor nanodisc self assembly by exploiting aggregation-caused-quenching (ACQ).

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Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs

Abstract

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Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

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Fingerprint

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Dataset for "Fluorescent Styrene Maleic Acid Copolymers to Facilitate Membrane Protein Studies in Lipid Nanodiscs"

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