Fluorescent small organic probes for biosensing

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Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity and sensitivity, whilst being non-invasive, and suitable for real-time analysis of living systems. With this perspective we highlight sensing mechanisms including Förster resonance energy transfer (FRET), intramolecular charge transfer (ICT), photoinduced electron transfer (PeT), excited state intramolecular proton transfer (ESIPT), aggregation induced emission (AIE) and multiple modality fluorescence approaches including dual/triple sensing mechanisms (DSM or TSM). Throughout the perspective we highlight the remaining challenges and suggest potential directions for development towards improved small-molecule fluorescent probes suitable for biosensing.

Original languageEnglish
Pages (from-to)3406-3426
JournalChemical Science
Issue number10
Early online date18 Jan 2021
Publication statusPublished - 14 Mar 2021

Bibliographical note

Funding Information:
XT wishes to thank the University of Bath for supporting her PhD work in the UK. LCM wishes to thank EPSRC for DTP PhD funding. LW wishes to thank China Scholarship Council and the University of Bath for supporting his PhD work in the UK. SEL wishes to thank EPSRC for funding (EP/R51164X/1). TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01).

Publisher Copyright:
© The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Chemistry(all)


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