Fluorescent small organic probes for biosensing

Xue Tian, Lloyd C. Murfin, Luling Wu, Simon E. Lewis, Tony D. James

Research output: Contribution to journalArticlepeer-review

23 Citations (SciVal)
38 Downloads (Pure)


Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity and sensitivity, whilst being non-invasive, and suitable for real-time analysis of living systems. With this perspective we highlight sensing mechanisms including Förster resonance energy transfer (FRET), intramolecular charge transfer (ICT), photoinduced electron transfer (PeT), excited state intramolecular proton transfer (ESIPT), aggregation induced emission (AIE) and multiple modality fluorescence approaches including dual/triple sensing mechanisms (DSM or TSM). Throughout the perspective we highlight the remaining challenges and suggest potential directions for development towards improved small-molecule fluorescent probes suitable for biosensing.

Original languageEnglish
Pages (from-to)3406-3426
JournalChemical Science
Issue number10
Early online date18 Jan 2021
Publication statusPublished - 14 Mar 2021

ASJC Scopus subject areas

  • Chemistry(all)


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