Fluorescent sensor for bacterial recognition

Rehab Amin, Souad A. Elfeky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Boronic acid-based fluorescent sensor is one of the non-enzymatic methods used for the recognition of saccharides. Since bacterial membrane has polysaccharides with diol groups, boronic acids probe could be applied for rapid bacterial recognition. Escherichia coli (XL-1 blue) were recognized by applying (3-(5-(dimethylamino) naphthalene-1-sulfonamide) phenyl) boronic acid (DNSBA) as a sensor and the fluorescence recorded by fluorometer micro-plate reader. Results showed that, fluorescence records of DNSBA increase in a dose dependent manner upon increasing the bacterial cell numbers. Moreover, the increase in the number of bacterial cells induces a shift in the spectra due to the formation of the anionic form of boronic acid complex. Therefore, DNSBA is an efficient sensor for monitoring bacterial cells.
Original languageEnglish
Pages (from-to)338-341
Number of pages4
JournalSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume108
DOIs
Publication statusPublished - May 2013

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