TY - JOUR
T1 - Fluorescent sensor for bacterial recognition
AU - Amin, Rehab
AU - Elfeky, Souad A.
PY - 2013/5
Y1 - 2013/5
N2 - Boronic acid-based fluorescent sensor is one of the non-enzymatic methods used for the recognition of saccharides. Since bacterial membrane has polysaccharides with diol groups, boronic acids probe could be applied for rapid bacterial recognition. Escherichia coli (XL-1 blue) were recognized by applying (3-(5-(dimethylamino) naphthalene-1-sulfonamide) phenyl) boronic acid (DNSBA) as a sensor and the fluorescence recorded by fluorometer micro-plate reader. Results showed that, fluorescence records of DNSBA increase in a dose dependent manner upon increasing the bacterial cell numbers. Moreover, the increase in the number of bacterial cells induces a shift in the spectra due to the formation of the anionic form of boronic acid complex. Therefore, DNSBA is an efficient sensor for monitoring bacterial cells.
AB - Boronic acid-based fluorescent sensor is one of the non-enzymatic methods used for the recognition of saccharides. Since bacterial membrane has polysaccharides with diol groups, boronic acids probe could be applied for rapid bacterial recognition. Escherichia coli (XL-1 blue) were recognized by applying (3-(5-(dimethylamino) naphthalene-1-sulfonamide) phenyl) boronic acid (DNSBA) as a sensor and the fluorescence recorded by fluorometer micro-plate reader. Results showed that, fluorescence records of DNSBA increase in a dose dependent manner upon increasing the bacterial cell numbers. Moreover, the increase in the number of bacterial cells induces a shift in the spectra due to the formation of the anionic form of boronic acid complex. Therefore, DNSBA is an efficient sensor for monitoring bacterial cells.
UR - http://www.scopus.com/inward/record.url?scp=84876032372&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.saa.2013.02.041
U2 - 10.1016/j.saa.2013.02.041
DO - 10.1016/j.saa.2013.02.041
M3 - Article
AN - SCOPUS:84876032372
SN - 1386-1425
VL - 108
SP - 338
EP - 341
JO - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
ER -