Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Wei Tao Dou, Li Fang Liu, Jie Gao, Yi Zang, Guo Rong Chen, Robert A. Field, Tony D. James, Jia Li, Xiao Peng He

Research output: Contribution to journalArticle

Abstract

Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.

Original languageEnglish
Pages (from-to)13235-13238
Number of pages4
JournalChemical communications (Cambridge, England)
Volume55
Issue number88
Early online date2 Oct 2019
DOIs
Publication statusPublished - 14 Nov 2019

Keywords

  • Biomarkers, Tumor/analysis
  • Cell Line, Tumor
  • Endothelial Protein C Receptor/analysis
  • Female
  • Fluorescent Dyes/chemistry
  • Humans
  • MCF-7 Cells
  • Molecular Structure
  • Optical Imaging
  • Particle Size
  • Peptides/chemistry
  • Spectrometry, Fluorescence
  • Surface Properties
  • Triple Negative Breast Neoplasms/diagnostic imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Chemistry(all)
  • Catalysis

Cite this