Floating lipid bilayers deposited on chemically grafted phosphatidylcholine surfaces

A V Hughes, J R Howse, A Dabkowska, R A L Jones, M J Lawrence, Stephen J Roser

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Floating supported bilayers (FSBs) are new systems which have emerged over the past few years to produce supported membrane mimics, where the bilayers remain associated with the substrate, but are cushioned from the substrates constraining influence by a large hydration layer. In this paper we describe a new approach to fabricating FSBs using a chemically grafted phospholipid layer as the support for the floating membrane. The grafted lipid layer. was produced using a Langmuir-Schaeffer transfer of acryloyl-functionalized lipid onto a pre-prepared substrate, with AIBN-induced cross-polymerization to permanently bind the lipids in place. A bilayer of DSPC was then deposited onto this grafted monolayer using a combination of Langmuir-Blodgett and Langmuir-Schaeffer transfer. The resulting system was characterized by neutron reflection under two water contrasts, and we show that the new system shows a hydrating layer of similar to 17.5 angstrom in the gel phase, which is comparable to previously described FSB systems. We provide evidence that the grafted substrate is reusable after cleaning and suggest that this greatly simplifies the fabrication and characterization of FSBs compared to previous methods.
Original languageEnglish
Pages (from-to)1989-1999
Number of pages11
JournalLangmuir
Volume24
Issue number5
Early online date17 Jan 2008
DOIs
Publication statusPublished - 4 Mar 2008

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