We synthesised analogues of diphosphoinositol polyphosphates (PP-InsPs) in which the diphosphate is replaced by an α-phosphonoacetic acid (PA) ester. Structural analysis revealed that 5-PA-InsP(5) mimics 5-PP-InsP(5) binding to the kinase domain of PPIP5K2; both molecules were phosphorylated by the enzyme. PA-InsPs are promising candidates for further studies into the biology of PP-InsPs.
Riley, A. M., Wang, H., Weaver, J. D., Shears, S. B., & Potter, B. V. L. (2012). First synthetic analogues of diphosphoinositol polyphosphates: interaction with PP-InsP5 kinase. Chemical Communications, 48(92), 11292-11294. https://doi.org/10.1039/c2cc36044f