First detection of porcine respirovirus 1 in Germany and the Netherlands

Leonard Schuele; Erley Lizarazo-Forero; Hayley Cassidy; Katrin Strutzberg-Minder; Jan Boehmer; Sabine Schuetze; Sandra Loebert; Claudia Lambrecht; Juergen Harlizius; Alex W. Friedrich; Silke Peter; John W.A. Rossen; Natacha Couto

doi:10.1111/tbed.14100

First detection of porcine respirovirus 1 in Germany and the Netherlands

Leonard Schuele, Erley Lizarazo-Forero, Hayley Cassidy, Katrin Strutzberg-Minder, Jan Boehmer, Sabine Schuetze, Sandra Loebert, Claudia Lambrecht, Juergen Harlizius, Alex W. Friedrich, Silke Peter, John W.A. Rossen, Natacha Couto

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

Abstract

Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch–German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.

Original language	English
Pages (from-to)	3120-3125
Number of pages	6
Journal	Transboundary and Emerging Diseases
Volume	68
Issue number	6
Early online date	5 May 2021
DOIs	https://doi.org/10.1111/tbed.14100
Publication status	Published - 30 Nov 2021

Bibliographical note

Funding Information:
The Food Pro‐tec‐ts project is carried out within the framework of the INTERREG V A programme 'Deutschland‐Nederland' and is co‐financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine‐Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein‐Waal. LS and HC received a grant from the Marie Skłodowska‐Curie Actions (Grant Agreement number: 713660—PRONKJEWAIL—H2020—MSCA‐COFUND‐2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

Funding Information:
The Food Pro-tec-ts project is carried out within the framework of the INTERREG V A programme 'Deutschland-Nederland' and is co-financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine-Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein-Waal. LS and HC received a grant from the Marie Sk?odowska-Curie Actions (Grant Agreement number: 713660?PRONKJEWAIL?H2020?MSCA-COFUND-2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We thank Erwin C. RaaNGS for expert technical support and the farmers for their participation in the study.

Publisher Copyright:
© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH

Keywords

Europe
next-generation sequencing
porcine parainfluenza virus 1
porcine respirovirus 1
shotgun metagenomics sequencing

ASJC Scopus subject areas

General Immunology and Microbiology
General Veterinary

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10.1111/tbed.14100Licence: CC BY

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Schuele, L., Lizarazo-Forero, E., Cassidy, H., Strutzberg-Minder, K., Boehmer, J., Schuetze, S., Loebert, S., Lambrecht, C., Harlizius, J., Friedrich, A. W., Peter, S., Rossen, J. W. A., & Couto, N. (2021). First detection of porcine respirovirus 1 in Germany and the Netherlands. Transboundary and Emerging Diseases, 68(6), 3120-3125. https://doi.org/10.1111/tbed.14100

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title = "First detection of porcine respirovirus 1 in Germany and the Netherlands",

abstract = "Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch–German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.",

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author = "Leonard Schuele and Erley Lizarazo-Forero and Hayley Cassidy and Katrin Strutzberg-Minder and Jan Boehmer and Sabine Schuetze and Sandra Loebert and Claudia Lambrecht and Juergen Harlizius and Friedrich, {Alex W.} and Silke Peter and Rossen, {John W.A.} and Natacha Couto",

note = "Funding Information: The Food Pro‐tec‐ts project is carried out within the framework of the INTERREG V A programme 'Deutschland‐Nederland' and is co‐financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine‐Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein‐Waal. LS and HC received a grant from the Marie Sk{\l}odowska‐Curie Actions (Grant Agreement number: 713660—PRONKJEWAIL—H2020—MSCA‐COFUND‐2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Funding Information: The Food Pro-tec-ts project is carried out within the framework of the INTERREG V A programme 'Deutschland-Nederland' and is co-financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine-Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein-Waal. LS and HC received a grant from the Marie Sk?odowska-Curie Actions (Grant Agreement number: 713660?PRONKJEWAIL?H2020?MSCA-COFUND-2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We thank Erwin C. RaaNGS for expert technical support and the farmers for their participation in the study. Publisher Copyright: {\textcopyright} 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH",

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AU - Cassidy, Hayley

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AU - Boehmer, Jan

AU - Schuetze, Sabine

AU - Loebert, Sandra

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AU - Harlizius, Juergen

AU - Friedrich, Alex W.

AU - Peter, Silke

AU - Rossen, John W.A.

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First detection of porcine respirovirus 1 in Germany and the Netherlands

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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