TY - JOUR
T1 - First detection of porcine respirovirus 1 in Germany and the Netherlands
AU - Schuele, Leonard
AU - Lizarazo-Forero, Erley
AU - Cassidy, Hayley
AU - Strutzberg-Minder, Katrin
AU - Boehmer, Jan
AU - Schuetze, Sabine
AU - Loebert, Sandra
AU - Lambrecht, Claudia
AU - Harlizius, Juergen
AU - Friedrich, Alex W.
AU - Peter, Silke
AU - Rossen, John W.A.
AU - Couto, Natacha
N1 - Funding Information:
The Food Pro‐tec‐ts project is carried out within the framework of the INTERREG V A programme 'Deutschland‐Nederland' and is co‐financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine‐Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein‐Waal. LS and HC received a grant from the Marie Skłodowska‐Curie Actions (Grant Agreement number: 713660—PRONKJEWAIL—H2020—MSCA‐COFUND‐2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
Funding Information:
The Food Pro-tec-ts project is carried out within the framework of the INTERREG V A programme 'Deutschland-Nederland' and is co-financed by the European Regional Development Fund (ERDF) and the Dutch Ministry of Economic Affairs and Climate Change, the Ministry of Economic Affairs, Innovation, Digitalisation and Energy of North Rhine-Westphalia and the provinces of Gelderland, Limburg and North Brabant. It is accompanied by the programme management at the Euregio Rhein-Waal. LS and HC received a grant from the Marie Sk?odowska-Curie Actions (Grant Agreement number: 713660?PRONKJEWAIL?H2020?MSCA-COFUND-2015). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We thank Erwin C. RaaNGS for expert technical support and the farmers for their participation in the study.
Publisher Copyright:
© 2021 The Authors. Transboundary and Emerging Diseases published by Wiley-VCH GmbH
PY - 2021/11/30
Y1 - 2021/11/30
N2 - Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch–German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.
AB - Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch–German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.
KW - Europe
KW - next-generation sequencing
KW - porcine parainfluenza virus 1
KW - porcine respirovirus 1
KW - shotgun metagenomics sequencing
UR - http://www.scopus.com/inward/record.url?scp=85104560225&partnerID=8YFLogxK
U2 - 10.1111/tbed.14100
DO - 10.1111/tbed.14100
M3 - Article
C2 - 33837672
AN - SCOPUS:85104560225
SN - 1865-1674
VL - 68
SP - 3120
EP - 3125
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
IS - 6
ER -