TY - JOUR
T1 - Field investigation with real-time virus genetic characterisation support of a cluster of Ebola virus disease cases in Dubréka, Guinea, April to june 2015
AU - Pini, Alessandro
AU - Zomahoun, Delayo
AU - Duraffour, Sophie
AU - Derrough, Tarik
AU - Charles, Myrna
AU - Quick, Joshua
AU - Loman, Nick
AU - Cowley, Lauren
AU - Leno, Mamadou
AU - Ouedraogo, Nobila
AU - Thiam, Oumou
AU - Hernandez-Romieu, Alfonso
AU - Iko, Annie
AU - Keita, Halimatou
AU - Konate, Djiba
AU - Aboubak Soumah, Aboubacar
AU - Bouchouar, Etran
AU - Ileka-Priouzeau, Samuel
AU - Keita, Sakoba
AU - Diallo, Boubacar
AU - Cisse, Fode
AU - Jansa, Josep
AU - Carroll, Miles
AU - Guenther, Stephan
AU - Severi, Ettore
AU - Formenty, Pierre
N1 - Publisher Copyright:
© 2018, European Centre for Disease Prevention and Control (ECDC). All rights reserved.
PY - 2022/3/22
Y1 - 2022/3/22
N2 - On 11 May 2015, the Dubréka prefecture, Guinea, reported nine laboratory-confirmed cases of Ebola virus disease (EVD). None could be epidemiologically linked to cases previously reported in the prefecture. We describe the epidemiological and molecular investigations of this event. We used the Dubréka EVD registers and the Ebola treatment centre’s (ETC) records to characterise chains of transmission. Real-time field Ebola virus sequencing was employed to support epidemiological results. An epidemiological cluster of 32 cases was found, of which 27 were laboratory confirmed, 24 were isolated and 20 died. Real-time viral sequencing on 12 cases demonstrated SL3 lineage viruses with sequences differing by one to three nt inside a single phylogenetic cluster. For isolated cases, the average time between symptom onset and ETC referral was 2.8 days (interquartile range (IQR): 1–4). The average time between sample collection and molecular results’ availability was 3 days (IQR: 2–5). In an area with scarce resources, the genetic characterization supported the outbreak investigations in real time, linking cases where epidemiological investigation was limited and reassuring that the responsible strain was already circulating in Guinea. We recommend coupling thorough epidemiological and genomic investigations to control EVD clusters.
AB - On 11 May 2015, the Dubréka prefecture, Guinea, reported nine laboratory-confirmed cases of Ebola virus disease (EVD). None could be epidemiologically linked to cases previously reported in the prefecture. We describe the epidemiological and molecular investigations of this event. We used the Dubréka EVD registers and the Ebola treatment centre’s (ETC) records to characterise chains of transmission. Real-time field Ebola virus sequencing was employed to support epidemiological results. An epidemiological cluster of 32 cases was found, of which 27 were laboratory confirmed, 24 were isolated and 20 died. Real-time viral sequencing on 12 cases demonstrated SL3 lineage viruses with sequences differing by one to three nt inside a single phylogenetic cluster. For isolated cases, the average time between symptom onset and ETC referral was 2.8 days (interquartile range (IQR): 1–4). The average time between sample collection and molecular results’ availability was 3 days (IQR: 2–5). In an area with scarce resources, the genetic characterization supported the outbreak investigations in real time, linking cases where epidemiological investigation was limited and reassuring that the responsible strain was already circulating in Guinea. We recommend coupling thorough epidemiological and genomic investigations to control EVD clusters.
UR - http://www.scopus.com/inward/record.url?scp=85044768438&partnerID=8YFLogxK
U2 - 10.2807/1560-7917.ES.2018.23.12.17-00140
DO - 10.2807/1560-7917.ES.2018.23.12.17-00140
M3 - Article
VL - 23
JO - Eurosurveillance
JF - Eurosurveillance
SN - 1560-7917
IS - 12
M1 - 17-00140
ER -