Fibre-based spectral ratio Endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence

Helen E. Parker, James M. Stone, Adam D.L. Marshall, Tushar R. Choudhary, Robert R. Thomson, Kevin Dhaliwal, Michael G. Tanner

Research output: Contribution to journalArticle

Abstract

Fibre-based optical endomicroscopy (OEM) permits high resolution fluorescence microscopy in endoscopically accessible tissues. Fibred OEM has the potential to visualise pathologies targeted with fluorescent imaging probes and provide an in vivo in situ molecular pathology platform to augment disease understanding, diagnosis and stratification. Here we present an inexpensive widefield ratiometric fibred OEM system capable of enhancing the contrast between similar spectra of pathologically relevant fluorescent signals without the burden of complex spectral unmixing. As an exemplar, we demonstrate the potential of the platform to detect fluorescently labelled Gram-negative bacteria in the challenging environment of highly autofluorescent lung tissue in whole ex vivo human lungs.

Original languageEnglish
Article number#355097
Pages (from-to)1856-1869
Number of pages14
JournalBiomedical Optics Express
Volume10
Issue number4
DOIs
Publication statusPublished - 1 Apr 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Parker, H. E., Stone, J. M., Marshall, A. D. L., Choudhary, T. R., Thomson, R. R., Dhaliwal, K., & Tanner, M. G. (2019). Fibre-based spectral ratio Endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence. Biomedical Optics Express, 10(4), 1856-1869. [#355097]. https://doi.org/10.1364/BOE.10.001856

Fibre-based spectral ratio Endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence. / Parker, Helen E.; Stone, James M.; Marshall, Adam D.L.; Choudhary, Tushar R.; Thomson, Robert R.; Dhaliwal, Kevin; Tanner, Michael G.

In: Biomedical Optics Express, Vol. 10, No. 4, #355097, 01.04.2019, p. 1856-1869.

Research output: Contribution to journalArticle

Parker, HE, Stone, JM, Marshall, ADL, Choudhary, TR, Thomson, RR, Dhaliwal, K & Tanner, MG 2019, 'Fibre-based spectral ratio Endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence', Biomedical Optics Express, vol. 10, no. 4, #355097, pp. 1856-1869. https://doi.org/10.1364/BOE.10.001856
Parker, Helen E. ; Stone, James M. ; Marshall, Adam D.L. ; Choudhary, Tushar R. ; Thomson, Robert R. ; Dhaliwal, Kevin ; Tanner, Michael G. / Fibre-based spectral ratio Endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 4. pp. 1856-1869.
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