Ferrocene-labelled electroactive aptamer-based sensors (aptasensors) for glycated haemoglobin

Xue Qing Feng; Yi Ju; Wei-Tao Dou; Qing Li; Zhong-Gan Jin; Xiao-Peng He; Tony James; Bang-Ce Ye

doi:10.3390/molecules26237077

Ferrocene-labelled electroactive aptamer-based sensors (aptasensors) for glycated haemoglobin

Xue Qing Feng, Yi Ju, Wei-Tao Dou, Qing Li, Zhong-Gan Jin, Xiao-Peng He, Tony James, Bang-Ce Ye

Research output: Contribution to journal › Article › peer-review

9 Citations (SciVal)

Abstract

Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c

Original language	English
Article number	7077
Journal	Molecules
Volume	26
Issue number	23
Early online date	23 Nov 2021
DOIs	https://doi.org/10.3390/molecules26237077
Publication status	Published - 1 Dec 2021

Funding

Funding: This research was funded by the National Natural Science Foundation of China (22107028), Natural Science Foundation of Shanghai (19ZR1445600), the Shanghai Science and Technology Committee (19410712600), National Postdoctoral Program for Innovative Talents (BX20190115), Shanghai Post-doctoral Excellence Program (2019044) and China Postdoctoral Science Foundation (2020M681206) for financial support. T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.3390/molecules26237077Licence: CC BY

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title = "Ferrocene-labelled electroactive aptamer-based sensors (aptasensors) for glycated haemoglobin",

abstract = "Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c",

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T1 - Ferrocene-labelled electroactive aptamer-based sensors (aptasensors) for glycated haemoglobin

AU - Feng, Xue Qing

AU - Ju, Yi

AU - Dou, Wei-Tao

AU - Li, Qing

AU - Jin, Zhong-Gan

AU - He, Xiao-Peng

AU - James, Tony

AU - Ye, Bang-Ce

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c

AB - Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c

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ER -

Ferrocene-labelled electroactive aptamer-based sensors (aptasensors) for glycated haemoglobin

Abstract

Funding

UN SDGs

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