Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

Yasemin L. Mustafa; Hannah S. Leese

doi:10.1021/acsomega.2c08127

Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

Yasemin L. Mustafa, Hannah S. Leese

Research output: Contribution to journal › Article › peer-review

10 Citations (SciVal)

Abstract

The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.

Original language	English
Pages (from-to)	8732-8742
Number of pages	11
Journal	ACS OMEGA
Volume	8
Issue number	9
DOIs	https://doi.org/10.1021/acsomega.2c08127
Publication status	Published - 7 Mar 2023

Bibliographical note

Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1.

Funding Information:
The authors gratefully acknowledge the Material and Chemical Characterization Facility (MC) at University of Bath (10.15125/mx6j-3r54). The authors thank the reviewers for their constructive feedback. The Table of Contents graphic, Figure 1, and Figure S2 were created with BioRender.com. This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1.

Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.

Access to Document

10.1021/acsomega.2c08127Licence: CC BY

Cite this

@article{169dfed8d5b64f56b4cd5ea44cdf09cd,

title = "Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection",

abstract = "The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.",

author = "Mustafa, {Yasemin L.} and Leese, {Hannah S.}",

note = "Funding Information: This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1. Funding Information: The authors gratefully acknowledge the Material and Chemical Characterization Facility (MC) at University of Bath (10.15125/mx6j-3r54). The authors thank the reviewers for their constructive feedback. The Table of Contents graphic, Figure 1, and Figure S2 were created with BioRender.com. This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

month = mar,

day = "7",

doi = "10.1021/acsomega.2c08127",

language = "English",

volume = "8",

pages = "8732--8742",

journal = "ACS OMEGA",

issn = "2470-1343",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

AU - Mustafa, Yasemin L.

AU - Leese, Hannah S.

N1 - Funding Information: This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1. Funding Information: The authors gratefully acknowledge the Material and Chemical Characterization Facility (MC) at University of Bath (10.15125/mx6j-3r54). The authors thank the reviewers for their constructive feedback. The Table of Contents graphic, Figure 1, and Figure S2 were created with BioRender.com. This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/3/7

Y1 - 2023/3/7

N2 - The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.

AB - The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.

UR - http://www.scopus.com/inward/record.url?scp=85148906845&partnerID=8YFLogxK

U2 - 10.1021/acsomega.2c08127

DO - 10.1021/acsomega.2c08127

M3 - Article

SN - 2470-1343

VL - 8

SP - 8732

EP - 8742

JO - ACS OMEGA

JF - ACS OMEGA

IS - 9

ER -

Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Minimally Invasive Molecularly Imprinted Conductive Nanoneedle Sensors

Cite this

Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Projects

Minimally Invasive Molecularly Imprinted Conductive Nanoneedle Sensors

Cite this