Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

Yasemin L. Mustafa, Hannah S. Leese

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)

Abstract

The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.

Original languageEnglish
Pages (from-to)8732-8742
Number of pages11
JournalACS OMEGA
Volume8
Issue number9
DOIs
Publication statusPublished - 7 Mar 2023

Bibliographical note

Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1.

Funding Information:
The authors gratefully acknowledge the Material and Chemical Characterization Facility (MC) at University of Bath (10.15125/mx6j-3r54). The authors thank the reviewers for their constructive feedback. The Table of Contents graphic, Figure 1, and Figure S2 were created with BioRender.com. This work was supported by the Engineering and Physical Sciences Research Council EP/V010859/1.

Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.

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