Extruded microfluidic immunoassays

A. I. Ferreira; Ana P. Castanheira; R. G. Chahin; Malcolm R, Mackley; Alexander D. Edwards; Nuno M. Reis

Extruded microfluidic immunoassays

A. I. Ferreira, Ana P. Castanheira, R. G. Chahin, Malcolm R, Mackley, Alexander D. Edwards, Nuno M. Reis

Department of Chemical Engineering

Research output: Chapter or section in a book/report/conference proceeding › Chapter or section

Abstract

This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.

Original language	English
Title of host publication	Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013
Editors	R. Zengerle
Publisher	Royal Society of Chemistry
Pages	982-984
ISBN (Print)	9780979806469
Publication status	Published - Oct 2013

Cite this

Ferreira, A. I., Castanheira, A. P., Chahin, R. G., Mackley, M. R., Edwards, A. D., & Reis, N. M. (2013). Extruded microfluidic immunoassays. In R. Zengerle (Ed.), Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013 (pp. 982-984). Royal Society of Chemistry.

Extruded microfluidic immunoassays. / Ferreira, A. I.; Castanheira, Ana P.; Chahin, R. G. et al.
Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013. ed. / R. Zengerle. Royal Society of Chemistry, 2013. p. 982-984.

Research output: Chapter or section in a book/report/conference proceeding › Chapter or section

@inbook{4a99287cef104f1f8ef83399dd1d6a3c,

title = "Extruded microfluidic immunoassays",

abstract = "This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.",

author = "Ferreira, {A. I.} and Castanheira, {Ana P.} and Chahin, {R. G.} and Mackley, {Malcolm R,} and Edwards, {Alexander D.} and Reis, {Nuno M.}",

year = "2013",

month = oct,

language = "English",

isbn = "9780979806469",

pages = "982--984",

editor = "R. Zengerle",

booktitle = "Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013",

publisher = "Royal Society of Chemistry",

address = "UK United Kingdom",

}

TY - CHAP

T1 - Extruded microfluidic immunoassays

AU - Ferreira, A. I.

AU - Castanheira, Ana P.

AU - Chahin, R. G.

AU - Mackley, Malcolm R,

AU - Edwards, Alexander D.

AU - Reis, Nuno M.

PY - 2013/10

Y1 - 2013/10

N2 - This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.

AB - This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.

M3 - Chapter or section

SN - 9780979806469

SP - 982

EP - 984

BT - Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013

A2 - Zengerle, R.

PB - Royal Society of Chemistry

ER -

Extruded microfluidic immunoassays

Abstract

Fingerprint

Cite this