Extruded microfluidic immunoassays

A. I. Ferreira, Ana P. Castanheira, R. G. Chahin, Malcolm R, Mackley, Alexander D. Edwards, Nuno M. Reis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.
Original languageEnglish
Title of host publicationProceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013
EditorsR. Zengerle
PublisherRoyal Society of Chemistry
Pages982-984
ISBN (Print)9780979806469
Publication statusPublished - Oct 2013

Fingerprint

Fluorine containing polymers
Immunosorbents
Microfluidics
Assays
Plastics
Syringes
Biomarkers
Enzymes
Interleukin-12
Costs
Interleukin-6
Cytokines
Fluids

Cite this

Ferreira, A. I., Castanheira, A. P., Chahin, R. G., Mackley, M. R., Edwards, A. D., & Reis, N. M. (2013). Extruded microfluidic immunoassays. In R. Zengerle (Ed.), Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013 (pp. 982-984). Royal Society of Chemistry.

Extruded microfluidic immunoassays. / Ferreira, A. I.; Castanheira, Ana P.; Chahin, R. G.; Mackley, Malcolm R,; Edwards, Alexander D.; Reis, Nuno M.

Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013. ed. / R. Zengerle. Royal Society of Chemistry, 2013. p. 982-984.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ferreira, AI, Castanheira, AP, Chahin, RG, Mackley, MR, Edwards, AD & Reis, NM 2013, Extruded microfluidic immunoassays. in R Zengerle (ed.), Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013. Royal Society of Chemistry, pp. 982-984.
Ferreira AI, Castanheira AP, Chahin RG, Mackley MR, Edwards AD, Reis NM. Extruded microfluidic immunoassays. In Zengerle R, editor, Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013. Royal Society of Chemistry. 2013. p. 982-984
Ferreira, A. I. ; Castanheira, Ana P. ; Chahin, R. G. ; Mackley, Malcolm R, ; Edwards, Alexander D. ; Reis, Nuno M. / Extruded microfluidic immunoassays. Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013. editor / R. Zengerle. Royal Society of Chemistry, 2013. pp. 982-984
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