This paper reports the miniaturisation of sandwich enzyme-linked immunosorbent assays (ELISA) using a meltextruded fluoropolymer MicroCapillary Film (MCF). A prototype 8-channel*10-microcapillaries device was used for quantitative detection a range of human biomarkers in 30 mm long fluoropolymer MCF strips. This represents a novel combination of ultra-low cost plastic microfluidic strips, low-cost optical detection, short incubation times, pre-loaded reagents and sequential fluid aspiration based on disposable plastic syringes. The observed limit of detection (LOD) was ∼lng/ml for PSA, and 10-60pg/ml for IL-lbeta, IL-6 and IL-12 inflammatory cytokines, typically with CVs ≤ 10% which is comparable with performance of optimised colorimetric microwell-based ELISA.
|Title of host publication||Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013|
|Publisher||Royal Society of Chemistry|
|Publication status||Published - Oct 2013|
Ferreira, A. I., Castanheira, A. P., Chahin, R. G., Mackley, M. R., Edwards, A. D., & Reis, N. M. (2013). Extruded microfluidic immunoassays. In R. Zengerle (Ed.), Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Black Forest, Germany, 27-31 October 2013 (pp. 982-984). Royal Society of Chemistry.