Extraction of phytochemicals from the pomegranate (Punica granatum L., Punicaceae) by reverse iontophoresis

Kieran Moore, Shaun B. Reeksting, Vimal Nair, Steve T. Pannakal, Nita Roy, Joan Eilstein, Sébastien Grégoire, M. Begoña Delgado-Charro, Richard H. Guy

Research output: Contribution to journalArticlepeer-review

Abstract

Plant metabolic profiling can provide a wealth of information regarding the biochemical status of the organism, but sample acquisition typically requires an invasive and/or destructive extraction process. Reverse iontophoresis (RI) imposes a small electric field across a biological membrane to substantially enhance the transport of charged and polar compounds and has been employed, in particular, to extract biomarkers of interest across human skin. The objective of this work was to examine the capability of RI to sample phytochemicals in a minimally invasive fashion in fructo (i.e., from the intact fruit). RI was principally used to extract a model, bioactive compound - specifically, ellagic acid - from the fruit peel of Punica granatum L. The RI sampling protocol was refined using isolated peel, and a number of experimental factors were examined and optimised, including preparation of the peel samples, the current intensity applied and the pH of the medium into which samples were collected. The most favourable conditions (3 mA current for a period of 1 hour, into a buffer at pH 7.4) were then applied to the successful RI extraction of ellagic acid from intact pomegranates. Multiple additional phytochemicals were also extracted and identified by liquid chromatography with tandem mass spectrometry (LC-MS/MS). A successful proof-of-concept has been achieved, demonstrating the capability to non-destructively extract phytochemicals of interest from intact fruit.

Original languageEnglish
Pages (from-to)11261-11268
Number of pages8
JournalRSC Advances
Volume13
Issue number17
Early online date11 Apr 2023
DOIs
Publication statusPublished - 31 Dec 2023

Bibliographical note

Funding Information:
This research was funded by L'Oréal R&I.

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint

Dive into the research topics of 'Extraction of phytochemicals from the pomegranate (Punica granatum L., Punicaceae) by reverse iontophoresis'. Together they form a unique fingerprint.

Cite this