Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 908-913 |
| Number of pages | 6 |
| Journal | European Journal of Pharmaceutics and Biopharmaceutics |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- Skin
- Amino acids
- Clinical chemistry
- Reverse iontophoresis
- Therapeutic monitoring
- Non-invasive
- Phenylalanine
Cite this
Extraction of amino acids by reverse iontophoresis: Simulation of therapeutic monitoring in vitro. / Sieg, A; Jeanneret, F; Fathi, M; Hochstrasser, D; Rudaz, S; Veuthey, J L; Guy, R H; Delgado-Charro, M B.
In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 70, No. 3, 2008, p. 908-913.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Extraction of amino acids by reverse iontophoresis: Simulation of therapeutic monitoring in vitro
AU - Sieg, A
AU - Jeanneret, F
AU - Fathi, M
AU - Hochstrasser, D
AU - Rudaz, S
AU - Veuthey, J L
AU - Guy, R H
AU - Delgado-Charro, M B
PY - 2008
Y1 - 2008
N2 - Reverse iontophoresis across the skin has been investigated as alternative, non-invasive method for clinical and therapeutic drug monitoring. This research investigated the reverse iontophoretic extraction of 19 amino acids present at clinically relevant levels in the subdermal compartment of an in vitro diffusion cell. Over a simulated, systemic concentration range of 0-500 mu M, the extraction of amino acids was linear. Charged amino acids were extracted towards the electrode of opposite polarity, while zwitterionic species were extracted to both anode and cathode with the latter predominating. The reverse iontophoretic extraction flux was a linear function of amino acid isoelectric point, reflecting the different contributions of electromigration and electroosmosis to electrotrans port. Overall, the results confirm the feasibility of monitoring amino acids at clinically relevant levels and provide an incentive for in vivo research to further explore the clinical potential of reverse iontophoresis for the non-invasive monitoring of amino acids.
AB - Reverse iontophoresis across the skin has been investigated as alternative, non-invasive method for clinical and therapeutic drug monitoring. This research investigated the reverse iontophoretic extraction of 19 amino acids present at clinically relevant levels in the subdermal compartment of an in vitro diffusion cell. Over a simulated, systemic concentration range of 0-500 mu M, the extraction of amino acids was linear. Charged amino acids were extracted towards the electrode of opposite polarity, while zwitterionic species were extracted to both anode and cathode with the latter predominating. The reverse iontophoretic extraction flux was a linear function of amino acid isoelectric point, reflecting the different contributions of electromigration and electroosmosis to electrotrans port. Overall, the results confirm the feasibility of monitoring amino acids at clinically relevant levels and provide an incentive for in vivo research to further explore the clinical potential of reverse iontophoresis for the non-invasive monitoring of amino acids.
KW - Skin
KW - Amino acids
KW - Clinical chemistry
KW - Reverse iontophoresis
KW - Therapeutic monitoring
KW - Non-invasive
KW - Phenylalanine
UR - http://www.scopus.com/inward/record.url?scp=54949154842&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.ejpb.2008.07.003
U2 - 10.1016/j.ejpb.2008.07.003
DO - 10.1016/j.ejpb.2008.07.003
M3 - Article
VL - 70
SP - 908
EP - 913
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
SN - 0939-6411
IS - 3
ER -