Extraction and quantification of amino acids in human stratum corneum in vivo

J- P. Sylvestre, C. C. Bouissou, R. H. Guy, M. B. Delgado-Charro

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Amino acid (AA) levels in stratum corneum (SC) are potential biomarkers of skin health while their systemic levels may be used to diagnose inherited metabolic diseases.
Objectives: To examine reverse iontophoresis, in human volunteers, as a minimally invasive tool to analyse AAs within the skin and subdermally.
Methods: In four volunteers, the amounts of iontophoretically extracted AAs were compared with those determined in the SC following repetitive tape stripping and with the plasma concentrations. Glucose levels, evaluated in the different compartments, were used as a control.
Results: SC concentrations of 13 essentially zwitterionic AAs were ∼100-fold higher than the respective plasma levels. Passive and reverse iontophoretic extraction for 4 h did not deplete the SC depot of AAs, a fact reinforced by postextraction tape stripping, which revealed that AAs remained in the SC at this time. In contrast, glucose was much less abundant in the SC and was fully and relatively quickly extracted by reverse iontophoresis.
Conclusions: It follows that reverse iontophoresis is useful for quantifying AAs in the SC and these data are highly correlated with levels obtained by tape stripping. However, reverse iontophoresis is impractical for the routine monitoring of AA plasma concentrations (unlike the situation for glucose, the skin reservoir of which is much smaller).
Original languageEnglish
Pages (from-to)458-465
Number of pages8
JournalBritish Journal of Dermatology
Volume163
Issue number3
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Cornea
Iontophoresis
Amino Acids
Glucose
Skin
Volunteers
Metabolic Diseases
Biomarkers
Health

Keywords

  • reverse iontophoresis
  • tape stripping
  • amino acids
  • stratum corneum

Cite this

Extraction and quantification of amino acids in human stratum corneum in vivo. / Sylvestre, J- P.; Bouissou, C. C.; Guy, R. H.; Delgado-Charro, M. B.

In: British Journal of Dermatology, Vol. 163, No. 3, 09.2010, p. 458-465.

Research output: Contribution to journalArticle

@article{271e68729f2b4fc88fe9f8fa21317445,
title = "Extraction and quantification of amino acids in human stratum corneum in vivo",
abstract = "Background: Amino acid (AA) levels in stratum corneum (SC) are potential biomarkers of skin health while their systemic levels may be used to diagnose inherited metabolic diseases. Objectives: To examine reverse iontophoresis, in human volunteers, as a minimally invasive tool to analyse AAs within the skin and subdermally. Methods: In four volunteers, the amounts of iontophoretically extracted AAs were compared with those determined in the SC following repetitive tape stripping and with the plasma concentrations. Glucose levels, evaluated in the different compartments, were used as a control. Results: SC concentrations of 13 essentially zwitterionic AAs were ∼100-fold higher than the respective plasma levels. Passive and reverse iontophoretic extraction for 4 h did not deplete the SC depot of AAs, a fact reinforced by postextraction tape stripping, which revealed that AAs remained in the SC at this time. In contrast, glucose was much less abundant in the SC and was fully and relatively quickly extracted by reverse iontophoresis. Conclusions: It follows that reverse iontophoresis is useful for quantifying AAs in the SC and these data are highly correlated with levels obtained by tape stripping. However, reverse iontophoresis is impractical for the routine monitoring of AA plasma concentrations (unlike the situation for glucose, the skin reservoir of which is much smaller).",
keywords = "reverse iontophoresis, tape stripping, amino acids, stratum corneum",
author = "Sylvestre, {J- P.} and Bouissou, {C. C.} and Guy, {R. H.} and Delgado-Charro, {M. B.}",
year = "2010",
month = "9",
doi = "10.1111/j.1365-2133.2010.09805.x",
language = "English",
volume = "163",
pages = "458--465",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Extraction and quantification of amino acids in human stratum corneum in vivo

AU - Sylvestre, J- P.

AU - Bouissou, C. C.

AU - Guy, R. H.

AU - Delgado-Charro, M. B.

PY - 2010/9

Y1 - 2010/9

N2 - Background: Amino acid (AA) levels in stratum corneum (SC) are potential biomarkers of skin health while their systemic levels may be used to diagnose inherited metabolic diseases. Objectives: To examine reverse iontophoresis, in human volunteers, as a minimally invasive tool to analyse AAs within the skin and subdermally. Methods: In four volunteers, the amounts of iontophoretically extracted AAs were compared with those determined in the SC following repetitive tape stripping and with the plasma concentrations. Glucose levels, evaluated in the different compartments, were used as a control. Results: SC concentrations of 13 essentially zwitterionic AAs were ∼100-fold higher than the respective plasma levels. Passive and reverse iontophoretic extraction for 4 h did not deplete the SC depot of AAs, a fact reinforced by postextraction tape stripping, which revealed that AAs remained in the SC at this time. In contrast, glucose was much less abundant in the SC and was fully and relatively quickly extracted by reverse iontophoresis. Conclusions: It follows that reverse iontophoresis is useful for quantifying AAs in the SC and these data are highly correlated with levels obtained by tape stripping. However, reverse iontophoresis is impractical for the routine monitoring of AA plasma concentrations (unlike the situation for glucose, the skin reservoir of which is much smaller).

AB - Background: Amino acid (AA) levels in stratum corneum (SC) are potential biomarkers of skin health while their systemic levels may be used to diagnose inherited metabolic diseases. Objectives: To examine reverse iontophoresis, in human volunteers, as a minimally invasive tool to analyse AAs within the skin and subdermally. Methods: In four volunteers, the amounts of iontophoretically extracted AAs were compared with those determined in the SC following repetitive tape stripping and with the plasma concentrations. Glucose levels, evaluated in the different compartments, were used as a control. Results: SC concentrations of 13 essentially zwitterionic AAs were ∼100-fold higher than the respective plasma levels. Passive and reverse iontophoretic extraction for 4 h did not deplete the SC depot of AAs, a fact reinforced by postextraction tape stripping, which revealed that AAs remained in the SC at this time. In contrast, glucose was much less abundant in the SC and was fully and relatively quickly extracted by reverse iontophoresis. Conclusions: It follows that reverse iontophoresis is useful for quantifying AAs in the SC and these data are highly correlated with levels obtained by tape stripping. However, reverse iontophoresis is impractical for the routine monitoring of AA plasma concentrations (unlike the situation for glucose, the skin reservoir of which is much smaller).

KW - reverse iontophoresis

KW - tape stripping

KW - amino acids

KW - stratum corneum

UR - http://www.scopus.com/inward/record.url?scp=77955903732&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1111/j.1365-2133.2010.09805.x

U2 - 10.1111/j.1365-2133.2010.09805.x

DO - 10.1111/j.1365-2133.2010.09805.x

M3 - Article

VL - 163

SP - 458

EP - 465

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 3

ER -