Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes

Mareike G. Posner, Abhishek Upadhyay, Aisha Alsheikh Abubaker, Tiago M. Fortunato, Dina Vara, Ilaria Canobbio, Stefan Bagby, Giordano Pula

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Extracellular fibrinogen-binding protein (Efb) from Staphylococcus aureus inhibits platelet activation, although its mechanism of action has not been established. In this study, we discovered that the N-terminal region of Efb (Efb-N) promotes platelet binding of fibrinogen and that Efb-N binding to platelets proceeds via two independent mechanisms: fibrinogen-mediated and fibrinogen-independent. By proteomic analysis of Efb-interacting proteins within platelets and confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-1 as novel Efb interaction partners. The interaction of both P-selectin and multimerin-1 with Efb is independent of fibrinogen. We focused on Efb interaction with P-selectin. Excess of P-selectin extracellular domain significantly impaired Efb binding by activated platelets, suggesting that P-selectin is the main receptor for Efb on the surface of activated platelets. Efb-N interaction with P-selectin inhibited P-selectin binding to its physiological ligand, P-selectin glycoprotein ligand-1 (PSGL-1), both in cell lysates and in cell-free assays. Because of the importance of P-selectin-PSGL-1 binding in the interaction between platelets and leukocytes, we tested human whole blood and found that Efb abolishes the formation of platelet-monocyte and platelet-granulocyte complexes. In summary, we present evidence that in addition to its documented antithrombotic activity, Efb can play an immunoregulatory role via inhibition of P-selectin-PSGL-1-dependent formation of platelet-leukocyte complexes.
LanguageEnglish
Pages2764-2776
Number of pages22
JournalJournal of Biological Chemistry
Volume291
Issue number6
Early online date1 Dec 2015
DOIs
StatusPublished - 5 Feb 2016

Fingerprint

Platelets
Fibrinogen
Staphylococcus aureus
Carrier Proteins
Leukocytes
Blood Platelets
P-Selectin
Proteins
Assays
Platelet Activation
Immunoblotting
Granulocytes
Proteomics
Monocytes
Blood

Keywords

  • Staphylococcus aureus
  • Efb
  • fibrinogen
  • platelet
  • neutrophil
  • P-selectin
  • monocyte
  • PSGL1

Cite this

Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes. / Posner, Mareike G.; Upadhyay, Abhishek; Alsheikh Abubaker, Aisha; Fortunato, Tiago M.; Vara, Dina; Canobbio, Ilaria ; Bagby, Stefan; Pula, Giordano.

In: Journal of Biological Chemistry, Vol. 291, No. 6, 05.02.2016, p. 2764-2776.

Research output: Contribution to journalArticle

Posner, Mareike G. ; Upadhyay, Abhishek ; Alsheikh Abubaker, Aisha ; Fortunato, Tiago M. ; Vara, Dina ; Canobbio, Ilaria ; Bagby, Stefan ; Pula, Giordano. / Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 6. pp. 2764-2776.
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AU - Posner, Mareike G.

AU - Upadhyay, Abhishek

AU - Alsheikh Abubaker, Aisha

AU - Fortunato, Tiago M.

AU - Vara, Dina

AU - Canobbio, Ilaria

AU - Bagby, Stefan

AU - Pula, Giordano

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N2 - Extracellular fibrinogen-binding protein (Efb) from Staphylococcus aureus inhibits platelet activation, although its mechanism of action has not been established. In this study, we discovered that the N-terminal region of Efb (Efb-N) promotes platelet binding of fibrinogen and that Efb-N binding to platelets proceeds via two independent mechanisms: fibrinogen-mediated and fibrinogen-independent. By proteomic analysis of Efb-interacting proteins within platelets and confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-1 as novel Efb interaction partners. The interaction of both P-selectin and multimerin-1 with Efb is independent of fibrinogen. We focused on Efb interaction with P-selectin. Excess of P-selectin extracellular domain significantly impaired Efb binding by activated platelets, suggesting that P-selectin is the main receptor for Efb on the surface of activated platelets. Efb-N interaction with P-selectin inhibited P-selectin binding to its physiological ligand, P-selectin glycoprotein ligand-1 (PSGL-1), both in cell lysates and in cell-free assays. Because of the importance of P-selectin-PSGL-1 binding in the interaction between platelets and leukocytes, we tested human whole blood and found that Efb abolishes the formation of platelet-monocyte and platelet-granulocyte complexes. In summary, we present evidence that in addition to its documented antithrombotic activity, Efb can play an immunoregulatory role via inhibition of P-selectin-PSGL-1-dependent formation of platelet-leukocyte complexes.

AB - Extracellular fibrinogen-binding protein (Efb) from Staphylococcus aureus inhibits platelet activation, although its mechanism of action has not been established. In this study, we discovered that the N-terminal region of Efb (Efb-N) promotes platelet binding of fibrinogen and that Efb-N binding to platelets proceeds via two independent mechanisms: fibrinogen-mediated and fibrinogen-independent. By proteomic analysis of Efb-interacting proteins within platelets and confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-1 as novel Efb interaction partners. The interaction of both P-selectin and multimerin-1 with Efb is independent of fibrinogen. We focused on Efb interaction with P-selectin. Excess of P-selectin extracellular domain significantly impaired Efb binding by activated platelets, suggesting that P-selectin is the main receptor for Efb on the surface of activated platelets. Efb-N interaction with P-selectin inhibited P-selectin binding to its physiological ligand, P-selectin glycoprotein ligand-1 (PSGL-1), both in cell lysates and in cell-free assays. Because of the importance of P-selectin-PSGL-1 binding in the interaction between platelets and leukocytes, we tested human whole blood and found that Efb abolishes the formation of platelet-monocyte and platelet-granulocyte complexes. In summary, we present evidence that in addition to its documented antithrombotic activity, Efb can play an immunoregulatory role via inhibition of P-selectin-PSGL-1-dependent formation of platelet-leukocyte complexes.

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