Abstract
Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 angstrom resolution. The crystal appeared to belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 angstrom, beta = 111.85 degrees, and is estimated to contain two molecules of Cwp19 per asymmetric unit.
| Original language | English |
|---|---|
| Pages (from-to) | 762-767 |
| Number of pages | 6 |
| Journal | Acta Crystallographica Section F-Structural Biology and Crystallization Communications |
| Volume | 67 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2011 |
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Kirby, J. M., Thiyagarajan, N., Roberts, A. K., Shone, C. C., & Acharya, K. R. (2011). Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67(7), 762-767. https://doi.org/10.1107/s1744309111016770