Expression of amylase and other pancreatic genes in Xenopus

M E Horb, J M W Slack

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To better understand the relationship between the endocrine and exocrine cell types in the Xenopus pancreas. we have cloned the Xenopus amylase cDNA and compared its expression profile with that of four other pancreatic markers: insulin. glucagon, elastase and trypsinogen. Our results demonstrate that the first pancreatic marker to be expressed is insulin. exclusively in the dorsal pancreas. These insulin-expressing cells form small groups which resemble islets, but no insulin is detected in the central pancreas until stage 47. In contrast. the exocrine markers, amylase, elastase and trypsinogen are first expressed only in the central pancreas beginning at stage 41: by stage 45 their expression extends into the dorsal pancreas, Glucagon, on the other hand, is not expressed in the pancreas until stage 45. In the endocrine cell clusters we do not find glucagon-expressing cells surrounding insulin-expressing cells, either in the tadpole or in the mature frog pancreas. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalMechanisms of Development
Volume113
Issue number2
Publication statusPublished - 2002

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Amylases
Xenopus
Pancreas
Insulin
Genes
Glucagon
Trypsinogen
Endocrine Cells
Pancreatic Elastase
Anura
Larva
Complementary DNA

Cite this

Expression of amylase and other pancreatic genes in Xenopus. / Horb, M E; Slack, J M W.

In: Mechanisms of Development, Vol. 113, No. 2, 2002, p. 153-157.

Research output: Contribution to journalArticle

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