Expression of a bacterial gene in plants mediated by infectious geminivirus DNA

A Ward, P Etessami, J E Stanley

Research output: Contribution to journalArticle

Abstract

A viable coat protein deletion mutant of cassava latent virus (CLV) DNA 1 has been isolated, suggesting that this geminivirus might be exploited as a gene replacement vector. An extensive deletion of 727 nucleotides within the coat protein gene renders DNA 1 non-infectious. Chimeric clones have been constructed in which the deleted coat protein open reading frame has been replaced by the coding region of the bacterial chloramphenicol acetyl transferase (CAT) gene. Infectivity is restored to DNA 1 when the CAT gene is inserted in either orientation, producing symptoms typical of CLV infection. The results demonstrate that the coat protein plays no essential role in virus spread throughout the host. Levels of CAT expression of 80 U/mg soluble protein occur in systemically infected Nicotiana benthamiana leaves when the CAT gene is fused in-frame to the amino terminus of the coat protein, providing a sensitive assay for viral DNA replication.

Original languageEnglish
Pages (from-to)1583-7
Number of pages5
JournalEMBO Journal
Volume7
Issue number6
Publication statusPublished - Jun 1988

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Geminiviridae
Bacterial Genes
Capsid Proteins
Chloramphenicol
Transferases
DNA
Manihot
Genes
DNA Viruses
Viral DNA
Virus Diseases
DNA Replication
Open Reading Frames
Tobacco
Nucleotides
Clone Cells
Viruses
Proteins

Keywords

  • Journal Article

Cite this

Expression of a bacterial gene in plants mediated by infectious geminivirus DNA. / Ward, A; Etessami, P; Stanley, J E.

In: EMBO Journal, Vol. 7, No. 6, 06.1988, p. 1583-7.

Research output: Contribution to journalArticle

Ward, A, Etessami, P & Stanley, JE 1988, 'Expression of a bacterial gene in plants mediated by infectious geminivirus DNA', EMBO Journal, vol. 7, no. 6, pp. 1583-7.
Ward, A ; Etessami, P ; Stanley, J E. / Expression of a bacterial gene in plants mediated by infectious geminivirus DNA. In: EMBO Journal. 1988 ; Vol. 7, No. 6. pp. 1583-7.
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