Exposure to endocrine disrupting chemicals in personal care products

Barbara Kasprzyk-Hordern, Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Antimicrobials and UV filters are used as additives in a broad range of personal care and consumer products such as soaps, cosmetics and disinfectants to protect against physicalchemical and biological agents. Unfortunately, some of them have been proven to have an endocrine disrupting activity, posing a threat to public health. Furthermore, due to their hydrophobic nature, chemicals in personal care products are potentially bioaccumulative. There are also concerns with regards to possible effects of antimicrobials in personal care products on the development of antimicrobial resistance. However very little is known about human exposure to these chemicals. In this work the biotransformation of 8 UV filters (4-benzylphenol, benzophenone-1, benzophenone-2, homosalate, 4,4’-dihydroxybenzophenone, ensulizole, octocrylene, 3benzylidene camphor) and 3 antimicrobials (4-chloro-3,5-dimethylphenol, 4-chloro-3methylephenol, chlorothymol,) was investigated with the aim of identifying human-specific metabolites suitable as biomarkers of exposure by conducting in vitro experiments with human liver subcellular fractions, followed by in-vivo studies in pooled urine and wastewater. Analysis of samples was performed utilising high resolution mass spectrometry (HRMS) coupled with high performance liquid chromatography (HPLC). Raw data extracted from the system were processed with MetID software (Advanced Chemistry Development, Inc., ACD/Labs, UK) for prediction of metabolite structures. As a result, for the first time, possible phases-I and II metabolites were identified and their presence in wastewater samples was observed suggesting that the impact of the exposure to antimicrobials, UV filters and many more chemicals might need to be reconsidered. Furthermore, we provide a new analytical approach based on a combination of in-vitro experiments and semitargeted wastewater screening for future metabolism and epidemiological studies.
Indicate
LanguageEnglish
Title of host publicationTesting the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference
StatusPublished - 2017
EventTesting the Waters 2017: 3rd International Conference on Wastewater-based Epidermology - Lisbon Congress Centre , Lisbon , Portugal
Duration: 26 Oct 201727 Oct 2017
http://score-cost.eu/network-activities/meetings/ttw2017/

Conference

ConferenceTesting the Waters 2017: 3rd International Conference on Wastewater-based Epidermology
CountryPortugal
CityLisbon
Period26/10/1727/10/17
Internet address

Fingerprint

Endocrine Disruptors
Metabolites
homosalate
Wastewater
Camphor
Consumer products
Soaps (detergents)
Cosmetics
Disinfectants
Biological Factors
High performance liquid chromatography
Biomarkers
Public health
Metabolism
Liver
Mass spectrometry
Screening
Experiments

Cite this

Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern (2017). Exposure to endocrine disrupting chemicals in personal care products. In Testing the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference

Exposure to endocrine disrupting chemicals in personal care products. / Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern.

Testing the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference. 2017.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern 2017, Exposure to endocrine disrupting chemicals in personal care products. in Testing the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference. Testing the Waters 2017: 3rd International Conference on Wastewater-based Epidermology , Lisbon , Portugal, 26/10/17.
Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern. Exposure to endocrine disrupting chemicals in personal care products. In Testing the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference. 2017
Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern. / Exposure to endocrine disrupting chemicals in personal care products. Testing the waters 2017 Wastewater-based epidemiology: current applications and future perspectives 3rd international conference. 2017.
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abstract = "Antimicrobials and UV filters are used as additives in a broad range of personal care and consumer products such as soaps, cosmetics and disinfectants to protect against physicalchemical and biological agents. Unfortunately, some of them have been proven to have an endocrine disrupting activity, posing a threat to public health. Furthermore, due to their hydrophobic nature, chemicals in personal care products are potentially bioaccumulative. There are also concerns with regards to possible effects of antimicrobials in personal care products on the development of antimicrobial resistance. However very little is known about human exposure to these chemicals. In this work the biotransformation of 8 UV filters (4-benzylphenol, benzophenone-1, benzophenone-2, homosalate, 4,4’-dihydroxybenzophenone, ensulizole, octocrylene, 3benzylidene camphor) and 3 antimicrobials (4-chloro-3,5-dimethylphenol, 4-chloro-3methylephenol, chlorothymol,) was investigated with the aim of identifying human-specific metabolites suitable as biomarkers of exposure by conducting in vitro experiments with human liver subcellular fractions, followed by in-vivo studies in pooled urine and wastewater. Analysis of samples was performed utilising high resolution mass spectrometry (HRMS) coupled with high performance liquid chromatography (HPLC). Raw data extracted from the system were processed with MetID software (Advanced Chemistry Development, Inc., ACD/Labs, UK) for prediction of metabolite structures. As a result, for the first time, possible phases-I and II metabolites were identified and their presence in wastewater samples was observed suggesting that the impact of the exposure to antimicrobials, UV filters and many more chemicals might need to be reconsidered. Furthermore, we provide a new analytical approach based on a combination of in-vitro experiments and semitargeted wastewater screening for future metabolism and epidemiological studies. Indicate",
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AU - Luigi Lopardo, David Adams, Anderew Cummins, Axel Rydevik, Barbara KasprzykHordern

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N2 - Antimicrobials and UV filters are used as additives in a broad range of personal care and consumer products such as soaps, cosmetics and disinfectants to protect against physicalchemical and biological agents. Unfortunately, some of them have been proven to have an endocrine disrupting activity, posing a threat to public health. Furthermore, due to their hydrophobic nature, chemicals in personal care products are potentially bioaccumulative. There are also concerns with regards to possible effects of antimicrobials in personal care products on the development of antimicrobial resistance. However very little is known about human exposure to these chemicals. In this work the biotransformation of 8 UV filters (4-benzylphenol, benzophenone-1, benzophenone-2, homosalate, 4,4’-dihydroxybenzophenone, ensulizole, octocrylene, 3benzylidene camphor) and 3 antimicrobials (4-chloro-3,5-dimethylphenol, 4-chloro-3methylephenol, chlorothymol,) was investigated with the aim of identifying human-specific metabolites suitable as biomarkers of exposure by conducting in vitro experiments with human liver subcellular fractions, followed by in-vivo studies in pooled urine and wastewater. Analysis of samples was performed utilising high resolution mass spectrometry (HRMS) coupled with high performance liquid chromatography (HPLC). Raw data extracted from the system were processed with MetID software (Advanced Chemistry Development, Inc., ACD/Labs, UK) for prediction of metabolite structures. As a result, for the first time, possible phases-I and II metabolites were identified and their presence in wastewater samples was observed suggesting that the impact of the exposure to antimicrobials, UV filters and many more chemicals might need to be reconsidered. Furthermore, we provide a new analytical approach based on a combination of in-vitro experiments and semitargeted wastewater screening for future metabolism and epidemiological studies. Indicate

AB - Antimicrobials and UV filters are used as additives in a broad range of personal care and consumer products such as soaps, cosmetics and disinfectants to protect against physicalchemical and biological agents. Unfortunately, some of them have been proven to have an endocrine disrupting activity, posing a threat to public health. Furthermore, due to their hydrophobic nature, chemicals in personal care products are potentially bioaccumulative. There are also concerns with regards to possible effects of antimicrobials in personal care products on the development of antimicrobial resistance. However very little is known about human exposure to these chemicals. In this work the biotransformation of 8 UV filters (4-benzylphenol, benzophenone-1, benzophenone-2, homosalate, 4,4’-dihydroxybenzophenone, ensulizole, octocrylene, 3benzylidene camphor) and 3 antimicrobials (4-chloro-3,5-dimethylphenol, 4-chloro-3methylephenol, chlorothymol,) was investigated with the aim of identifying human-specific metabolites suitable as biomarkers of exposure by conducting in vitro experiments with human liver subcellular fractions, followed by in-vivo studies in pooled urine and wastewater. Analysis of samples was performed utilising high resolution mass spectrometry (HRMS) coupled with high performance liquid chromatography (HPLC). Raw data extracted from the system were processed with MetID software (Advanced Chemistry Development, Inc., ACD/Labs, UK) for prediction of metabolite structures. As a result, for the first time, possible phases-I and II metabolites were identified and their presence in wastewater samples was observed suggesting that the impact of the exposure to antimicrobials, UV filters and many more chemicals might need to be reconsidered. Furthermore, we provide a new analytical approach based on a combination of in-vitro experiments and semitargeted wastewater screening for future metabolism and epidemiological studies. Indicate

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