Abstract
Aims: Exercise increases beta cell health in people at risk of and with established Type 2 diabetes. These benefits of exercise have not been characterised in Type 1 diabetes. Over 10% of beta cells are still present at the time of diagnosis with Type 1 diabetes, and exercise has the potential to preserve them. We aimed to explore the mechanisms through which exercise could improve beta cell health in Type 1 diabetes by investigating the effects of exercise serum on apoptosis and proliferation of the MIN6 mouse insulinoma cell line. Methods: Fasted blood was taken from 10 healthy male subjects before and after 40 min of varied unsupervised moderate intensity exercise, and from 11 well-trained male subjects before and after a 9 day intensive cycling exercise training study. Apoptosis was measured in MIN6 (24 h + 100 μM H2O2) by flow cytometry (AnnexinV-FITC, 7-AAD staining). Proliferation of MIN6 was measured using Promega CellTiter 96 Aqueous One Cell proliferation assay daily over 4 days. Experimental cultures were supplemented with 10% of either pre- or post-exercise serum. Results: MIN6 incubated in post-exercise serum showed 6.2% (SD ± pre, 12.88; post, 7.39) reduced apoptosis (p = 0.03) and 9% (SD ± pre, 0.339; post, 0.237) increased proliferation (p = 0.002) compared to those incubated in pre-exercise serum, and further increased proliferation by 43% (SD ± pre, 0.256; post, 0.367) with serum following 9 days' intensive training (p <0.001). Summary: Our results suggest exercise protects beta cells from apoptosis and increases their proliferation. Further benefits of exercise on beta cell health and the mechanisms through which they manifest in Type 1 diabetes need characterisation. This provides a basis to explore exercise as a potential therapy for patients with Type 1 diabetes.
Original language | English |
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Pages (from-to) | 76 |
Number of pages | 1 |
Journal | Diabetic Medicine |
Volume | 33 |
Issue number | Suppl. 1 |
DOIs | |
Publication status | Published - 1 Mar 2016 |
Keywords
- fish oil
- hydrogen peroxide
- fluorescein isothiocyanate
- health
- insulin dependent diabetes mellitus
- diabetes mellitus
- United Kingdom
- exercise
- human
- serum
- apoptosis
- male
- cell proliferation assay
- therapy
- staining
- flow cytometry
- mouse
- diagnosis
- blood
- insulinoma cell line
- non insulin dependent diabetes mellitus
- patient
- risk